表达p53基因的人胰腺癌细胞系JF305的建立  

ESTABLISHMENT OF A HUMAN PANCREATICADENOCARCINOMA CELL LINE(JF305)WITH p53 EXPRESSION

作　　者：李昕[1] 王宏[1] 姜奕[1] 王晓华[1] 贾兰玲[1] 张宝庚[1] 

机构地区：[1]中国医科大学肿瘤研究所

出　　处：《中华肿瘤杂志》1994年第3期181-183,T009,共4页Chinese Journal of Oncology

摘　　要：利用自建的人胰腺癌裸鼠移殖瘤，成功地建立了人胰腺癌细胞系ＪＦ３０５，历时一年余，２～３天传代一次，至今传代１０３次，平均倍增时间为４９．５小时，集落形成率为３０．６％，多次冻存、复苏，细胞生长稳定。重新接种于裸鼠皮下后形成移植瘤的生物学及形态学特征与该细胞系所来源的肿瘤相似，为中分化腺癌。染色体分析为人类染色体核型，数目在６６～１３０，免疫细胞化学染色发现ＪＦ３０５有ＣＥＡ和ｐ５３抑癌基因蛋白的过度表达。因此，ＪＦ３０５是人类胰腺癌研究的一个新的体内、体外模型系统，并为ｐ５３基因突变的研究提供了良好的实验材料。Abstract A human pancreatic cancer cell line (JF305) was established from a pancreatic adenocarcinoma xenograft in nude mice. These cells have passed 103 passages in more than 1 year. The plating effi-ciency was 30.6%, and doubling time was 49.5 hours. Chromosomal analysis showed a humankaryotype with a chromosome number between 66～130.JF305 cells exhibited epithelialmorphologic featurtes resembling the structure of the original tumor, and they grew to born tumorwhen inocuated again into nude mice. With immunohistochemical staining, the nuclei of JF305cells were positive for p53.Therefore, JF305 offer a good model for the study of humanpancreatic adenocarcinoma and p53 gene mutation.

关 键 词：胰腺肿瘤 基因表达 细胞株 

分 类 号：R735.9[医药卫生—肿瘤]