Smad7在人慢性髓细胞白血病细胞株K562中的表达  

Study on the expression of Smad7 in human chronic myelogenous leukemia cell line( K562)

作　　者：陈洁[1] 董戴玉[1] 杨镜明[1] 周欣[1] 

机构地区：[1]上海第二医科大学附属新华医院血液内科,上海200092

出　　处：《临床血液学杂志》2005年第4期229-231,共3页Journal of Clinical Hematology

摘　　要：目的:观察人慢性髓细胞白血病细胞株K562中Smad7基因和蛋白的表达;探讨转化生长因子(TGF-β1)对Smad7的诱导作用。方法:采用逆转录-聚合酶链反应、蛋白印迹法方法检测K562细胞与TGF-β1共育前后Smad7mRNA及蛋白表达水平。结果:K562细胞中具有Smad7的表达,且可由外源性TGF-β1短暂诱导,Smad7的表达及变化在转录及翻译水平上基本保持一致。结论:Smad7参与K562细胞TGF-β信号的传导,Smad7与TGF-β之间存在自身调节负反馈通路。Objective:To examine the expression of Smad7 gene and protein in human chronic myelogenous leukemia cell line (K562) and to explore the effects of TGF-β1 on the expression of Smad7 preliminaryly. Method:We studied the expression of Smad7 gene and protein in K562 cell line and the alteration of Smad7 after incubated with TGF-β1 by RT-PCR analysis, western blot. Result:The expression of Smad7 was detected in K562 cell line,and can be induced by the stimulation of TGF-β1 transiently. The expression of Smad7 and the alteration of Smad7 after TGF-β1 treatment at the translational level generally parallel to those at the transcriptional level. Conclusion:Smad7 Participates in the TGF-β signal transduction of K562 cell line.There's a negative auto-regulatory feedback loop between Smad7 and TGF-β1.

关 键 词：信号转导 慢性 白血病 

分 类 号：R733.72[医药卫生—肿瘤]