人雌激素受体相关受体逆转录病毒载体的构建及鉴定  

The construction and determination of the retroviral vector expressing human estrogen receptor-related receptor α

作　　者：高敏[1] 孙蓬明[1] 赵丹[1] 王建六[1] 魏丽惠[1] 

机构地区：[1]北京大学人民医院妇科,100044

出　　处：《中国妇产科临床杂志》2005年第4期275-277,共3页Chinese Journal of Clinical Obstetrics and Gynecology

基　　金：国家自然科学基金(30371477);首都医学发展科研基金(首都ZD199911)资助

摘　　要：目的构建人雌激素受体相关受体α(hERRα)逆转录病毒载体。方法用EcoRⅠ和BamHⅠ双酶切pSG-hERRα质粒和pLXSN空白质粒,采用凝胶DNA回收方法回收纯化hERRα片段,用T4连接酶进行连接反应,构建成PLXSN-neo-hERRα重组体。结果经PCR、酶切鉴定、测序等对重组体进行鉴定,证实构建的重组体方向、序列正确。结论已成功构建hERRα逆转录病毒载体,为研究hERRα的功能奠定了基础。Objective To construct the retroviral vector expressing the human estrogen receptor-related receptor. Methods The pSG-hERR α plasmid was digested by EcoR Ⅰ and BamHⅠ. The hERR α fragment was purified from gel and cloned into PLXSN-neo plasmid. The recombinant pLXSN-neo-hERR α plasmid was screened and determined by PCR, restriction enzymes digestion and DNA sequencing. Results The DNA sequence of pLXSN-neo-hERR α plasmid was confirmed by DNA sequencing. Conclusion PLXSN-neo-hERR α plasmid was constructed successfully which will serve in the research of hERR α function.

关 键 词：人雌激素受体相关受体 逆转录病毒载体 构建 鉴定 基因重组 

分 类 号：R737.33[医药卫生—肿瘤]