Expression of pro-inflammatory and anti-inflammatory cytokines in brain of atherosclerotic rats and effects of Ginkgo biloba extract  

作　　者：Ya-binJIAO Yao-chengRUI Tie-junLI Peng-yuanYANG YanQIU 

机构地区：[1]DepartmentofPharmacology,SchoolofPharmacy,theSecondMilitaryMedicalUniversityShanghai200433,China [2]NowinDepartmentofPharmacology,SchoolofPharmacy,ShanghaiUniversityofTraditionalChineseMedicine,Shanghai201203,China

出　　处：《Acta Pharmacologica Sinica》2005年第7期835-839,共5页中国药理学报（英文版）

基　　金：the National Natural Science Foundation of China(No 30271509).

摘　　要：Aim:To study the protein and mRNA expressions of pro-inflammatory and anti- inflammatory cytokines in the brain of rats with atherosclerosis(AS)and the effects of Ginkgo biloba extract(GbE)on expressions of cytokines.Methods: The expermental model of AS in rats were established by intraperitioneal injection of vitamin D3 with high fat/cholesterol diet.GbE 100 mg/kg was administered to rats by ig.After 8 weeks,the expressions of IL-1β,TNF-α,IL-10,and 1L-10R in the brain tissues of AS rats were detected by enzyme-linked immunosorbant assay, immunohistochemistry,Western blotting,and reverse transcriptase polymerase chain reaction.Results:The protein and mRNA expressions of IL-1β,TNF-α,and IL-10 in the brains were markedly higher in AS groups than that in control groups (6.11±0.15,1.55±0.14,0.54±0.04 ng/g wet weight vs 0.80±0.14,0.33±0.09,and 0.33±0.02 ng/g wet weight,respectively).The protein and mRNA expres- sions of IL-1β and TNF-α in the brains were markedly lower in GbE groups (3.82±0.54,0.95±0.08 ng/g wet weight)than that in AS groups,the protein and mRNA expressions of IL-10 and 1L-10R in the brains were markedly higher in GbE groups(0.85±0.06 ng/g wet weight)than that in AS groups.Conclusion:GbE inhibited production of pro-inflammatory cytokines IL-1β and TNF-α,but up- regulated the production of anti-inflammatory cytokines,1L-10 and IL-10R in brain, which might be related with its anti-AS actions.Aim:To study the protein and mRNA expressions of pro-inflammatory and anti- inflammatory cytokines in the brain of rats with atherosclerosis(AS)and the effects of Ginkgo biloba extract(GbE)on expressions of cytokines.Methods: The expermental model of AS in rats were established by intraperitioneal injection of vitamin D3 with high fat/cholesterol diet.GbE 100 mg/kg was administered to rats by ig.After 8 weeks,the expressions of IL-1β,TNF-α,IL-10,and 1L-10R in the brain tissues of AS rats were detected by enzyme-linked immunosorbant assay, immunohistochemistry,Western blotting,and reverse transcriptase polymerase chain reaction.Results:The protein and mRNA expressions of IL-1β,TNF-α,and IL-10 in the brains were markedly higher in AS groups than that in control groups (6.11±0.15,1.55±0.14,0.54±0.04 ng/g wet weight vs 0.80±0.14,0.33±0.09,and 0.33±0.02 ng/g wet weight,respectively).The protein and mRNA expres- sions of IL-1β and TNF-α in the brains were markedly lower in GbE groups (3.82±0.54,0.95±0.08 ng/g wet weight)than that in AS groups,the protein and mRNA expressions of IL-10 and 1L-10R in the brains were markedly higher in GbE groups(0.85±0.06 ng/g wet weight)than that in AS groups.Conclusion:GbE inhibited production of pro-inflammatory cytokines IL-1β and TNF-α,but up- regulated the production of anti-inflammatory cytokines,1L-10 and IL-10R in brain, which might be related with its anti-AS actions.

关 键 词：atherosclerosis cytokine interleukin-1 tumor necrosis factor interleukin-10 Ginkgo biloba RATS 

分 类 号：R743.1[医药卫生—神经病学与精神病学]