Genistein inhibits carotid sinus baroreceptor activity in anesthetized male rats  

作　　者：Hui-juanMA Yi-xianLIU Fu-weiWANG Li-xuanWANG Rui-rongHE Yu-mingWU 

机构地区：[1]DepartmentofPhysiology,InstituteofBasicMedicine,HebeiMedicalUniversity,Shijiazhuang050017,China

出　　处：《Acta Pharmacologica Sinica》2005年第7期840-844,共5页中国药理学报（英文版）

基　　金：a grant from Natural Science Foundation of Hebei Province of China(No 303467);National Natural Science Foundation of China(No 304005).

摘　　要：Aim:To study the effect of genistein(GST)on carotid baroreceptor activity (CBA).Methods:The functional curve of carotid baroreceptor(FCCB)was con- structed and the functional parameters of carotid baroreceptor were measured by recording sinus nerve afferent discharge in anesthetized male rats with perfused isolated carotid sinus.Results:GST at 50,100,and 200 μmol/L inhibited the CBA,which shifted FCCB to the fight and downward,with a marked decrease in peak slope and peak integral value of carotid sinus nerve discharge in a concen- tration-dependent manner.Pretreatment with 100 μmol/L N^G-nitro-L-arginine me- thyl ester,an inhibitor of nitric oxide synthase,did not affect the effect of GST on CBA.Pretreatment with 500 nmol/L Bay K8644,an agonist of calcium channels, could completely abolish the effect of GST on CBA.A potent inhibitor of tyrosine phosphatase,sodium orthovanadate(1 mmol/L),could attenuate the inhibitory effect of GST.Conclusion:GST inhibits CBA,and the effect may be mediated by protein tyrosine kinase inhibition and a decrease in Ca^(2+)influx through the stretch- activated channels.Aim:To study the effect of genistein(GST)on carotid baroreceptor activity (CBA).Methods:The functional curve of carotid baroreceptor(FCCB)was con- structed and the functional parameters of carotid baroreceptor were measured by recording sinus nerve afferent discharge in anesthetized male rats with perfused isolated carotid sinus.Results:GST at 50,100,and 200 μmol/L inhibited the CBA,which shifted FCCB to the fight and downward,with a marked decrease in peak slope and peak integral value of carotid sinus nerve discharge in a concen- tration-dependent manner.Pretreatment with 100 μmol/L N^G-nitro-L-arginine me- thyl ester,an inhibitor of nitric oxide synthase,did not affect the effect of GST on CBA.Pretreatment with 500 nmol/L Bay K8644,an agonist of calcium channels, could completely abolish the effect of GST on CBA.A potent inhibitor of tyrosine phosphatase,sodium orthovanadate(1 mmol/L),could attenuate the inhibitory effect of GST.Conclusion:GST inhibits CBA,and the effect may be mediated by protein tyrosine kinase inhibition and a decrease in Ca^(2+)influx through the stretch- activated channels.

关 键 词：GENISTEIN carotid sinus BAROREFLEX proteintyrosine kinase Bay K8644 L-NAME 

分 类 号：R543.4[医药卫生—心血管疾病]