Tetramethylpyrazine protected photoreceptor cells of rats by modulating nuclear translocation of NF-κB  

作　　者：Jin-nanYANG Jin-maoCHEN LinLUO Shao-chunLIN DaiLI Shi-xingH 

机构地区：[1]SchoolofPharmacy,XinxiangMedicalCollege,Xinxiang453003,China [2]ZhongshanOphthalmicCentre,SunYat-senUniversity,Guangzhou510060,China [3]ZhongshanOphthalmicCentre,SunYat-senUniversity,Guangzhou510060,China//EyeHospitalofChina,AcademyofTraditionalChineseMedicine,Beijing100040,China

出　　处：《Acta Pharmacologica Sinica》2005年第7期887-892,共6页中国药理学报（英文版）

基　　金：the National Natural Science Foundation of China,No 30300467.

摘　　要：Aim:To evaluate the effect of tetramethylpyrazine(TMP)injection on retinal damage induced by N-methyl-N-nitrosourea(MNU)in rats and on nuclear factor- kappa B(NF-κB)family members.Methods:Female Sprague-Dawley(SD)rats were randomly divided into groups:(i),control group;(ii),model group;and(iii), TMP-injection groups,in which the rats were subdivided into 40 mg/kg,80 mg/kg and 160 mg/kg groups.Drugs were injected ip into 47-day-old SD rats once a day. At 50 days of age,all rats in the model group and drug groups also received a single ip injection of 60 mg/kg MNU.Rats in group 1 received ip injection of physiological saline.All rats were killed at different times after MNU or physi- ological saline treatment.The apoptotic index of photoreceptor cells was calcu- lated by TUNEL labeling;retinal damage was evaluated based on retinal thickness and the expression of NF-κB family members was detected by Western blot. Results:TMP injections,in a dose-dependent manner,suppressed photorecep- tor cell apoptosis and decreased its loss in the peripheral retina.As compared with the MNU-treated group,TMP injection at a dose of 160 mg/kg also time- dependently upregulated the NF-κB/p65 protein level in the nucleus and downregulated the IκBα protein level in the cytoplasm.However,no protective effect of TMP injection on MNU-induced central retinal damage was found. Conclusion:TMP injection partially protects against MNU-induced retinal dam- age by upregulating the nuclear translocation of p65 to inhibit photoreceptor cells apoptosis.Aim:To evaluate the effect of tetramethylpyrazine(TMP)injection on retinal damage induced by N-methyl-N-nitrosourea(MNU)in rats and on nuclear factor- kappa B(NF-κB)family members.Methods:Female Sprague-Dawley(SD)rats were randomly divided into groups:(i),control group;(ii),model group;and(iii), TMP-injection groups,in which the rats were subdivided into 40 mg/kg,80 mg/kg and 160 mg/kg groups.Drugs were injected ip into 47-day-old SD rats once a day. At 50 days of age,all rats in the model group and drug groups also received a single ip injection of 60 mg/kg MNU.Rats in group 1 received ip injection of physiological saline.All rats were killed at different times after MNU or physi- ological saline treatment.The apoptotic index of photoreceptor cells was calcu- lated by TUNEL labeling;retinal damage was evaluated based on retinal thickness and the expression of NF-κB family members was detected by Western blot. Results:TMP injections,in a dose-dependent manner,suppressed photorecep- tor cell apoptosis and decreased its loss in the peripheral retina.As compared with the MNU-treated group,TMP injection at a dose of 160 mg/kg also time- dependently upregulated the NF-κB/p65 protein level in the nucleus and downregulated the IκBα protein level in the cytoplasm.However,no protective effect of TMP injection on MNU-induced central retinal damage was found. Conclusion:TMP injection partially protects against MNU-induced retinal dam- age by upregulating the nuclear translocation of p65 to inhibit photoreceptor cells apoptosis.

关 键 词：TETRAMETHYLPYRAZINE photoreceptors methylnitrosourea APOPTOSIS NF-kappa B retinitis pigmentosa 

分 类 号：R774.1[医药卫生—眼科] R988.1[医药卫生—临床医学]