腹腔注射顺氯氨铂后大鼠中枢神经系统内Fos蛋白的表达  

THE EXPRESSION OF FOS-LIKE IMMUNOREACTIVITY NEURONS IN THE CENTRAL NERVOUS SYSTEM IN THE RAT AFTER INTRAPERITONEAL INJECTION OF CISPLATIN

作　　者：孔哲[1] 季淑梅[2] 高萱[1] 陈应城[3] 管振龙[1] 

机构地区：[1]河北师范大学生命科学学院,石家庄050016 [2]河北医科大学基础医学研究所生理室,石家庄050017 [3]香港大学医学院生理学系

出　　处：《神经解剖学杂志》2005年第3期276-280,共5页Chinese Journal of Neuroanatomy

基　　金：河北省自然科学基金(No.303153)资助项目

摘　　要：为了研究非呕吐动物大鼠是否存在与呕吐动物相似的呕吐反应区,以及二者之间的异同,给予大鼠腹腔催吐剂-顺氯氨铂后,应用免疫组织化学方法,观察Fos阳性神经元在脑和脊髓内呕吐相关区域的分布。结果发现,在脑干的最后区、孤束核、外侧臂旁核和下丘脑的视上核、室旁核、弓状核有大量的Fos阳性神经元,实验组和对照组有显著性差异(P<0.05)。结论催吐剂的刺激可使大鼠脑内Fos阳性神经元数量增加,除了与呕吐运动相关的部分区域外,其余分布区域均与呕吐动物一致,提示大鼠脑内也存在类似的、与恶心相关的神经化学通路。To study whether there is similar emetic reflex area with non-emetic animals of rat and the similarity and difference between them, we used immnohistochemical technique to study the distribution of Fos-like immunoreactivity (Fos-LI) neurons in the nucleus of brain and spinal cord which are relate to emetic after intraperitoneal injection of emetic drug(cisplatin) in the rat. The results indicated that there were abundant Fos-LI neurons in area postrema, nuclei of the solitary tract, lateral parabrachial nuclei, supraoptic nucleus, paraventricular nucleus, arcuate nucleus, significant differences were observed between experimental and control groups in number of immunolabeled neurons(P< 0.05).Conclusion: The stimulation of emetic increase the number of Fos-LI neurons in rat brain, except the several area correlated with vomiting motion, the rest distribution areas are identical with emetic animals. The results suggest that there is similar emetic nerval-chemist pathway in the rat.

关 键 词：腹腔注射 顺氯氨铂 大鼠 中枢神经系统 FOS蛋白 呕吐反应 催吐剂 

分 类 号：R741[医药卫生—神经病学与精神病学]