海洛因依赖者Th1/Th2类细胞因子的变化  

The Changes of Th1/Th2 Type Cytokines in Heroin Addicts

作　　者：和虹[1] 杨丽萍[2] 杨国军[1] 邵宗鸿[3] 陈萍[1] 杨桂益 李志东 宋鲁燕[3] 储榆林[3] 

机构地区：[1]云南省大理学院,大理6710002 [2]河北省沧州市中心医院 [3]中国医学科学院血液学研究所 [4]云南大理市第一人民医院 [5]云南大理市强制戒毒所

出　　处：《中国药物滥用防治杂志》2005年第3期131-133,共3页Chinese Journal of Drug Abuse Prevention and Treatment

基　　金：国家自然科学基金资助项目(30060075);云南省教育委员会基金资助项目(0041068)

摘　　要：目的:研究Th1/Th2类细胞因子在海洛因依赖者中的变化,了解海洛因依赖者的免疫学状况。方法:利用细胞因子诱生技术及酶联免疫吸附试验(ELISA)检测30例海洛因依赖者及24名正常人外周血单个核细胞PBMC,体外刺激培养上清中Th1细胞的代表性细胞因子IFN-酌、IL-2及Th2细胞的代表性细胞因子IL-4、IL-6的分泌,比较海洛因依赖者与正常人上述细胞因子水平的差异。结果:海洛因依赖者IL-2、IFN-酌水平降低,而IL-4、IL-6水平增高,与正常对照比较有显著性差异。结论:海洛因依赖者存在Th1/Th2功能紊乱,主要表现为Th1细胞功能降低,Th2细胞功能亢进,这可能是海洛因依赖者感染性疾病发生率高的重要原因之一。Objective: To detect Th1/Th2 type cytokines profiles in heroin addicts, and compare the difference of immune status between heroin addicts and normal. Methods: Concentrations of IL-2, IFN-γ, IL-4 and IL-6 in culture supernatants of PMA and ionomycin stimulated peripheral blood mononuclear cells (PBMC) were measured by enzyme-linked immunosorbent assay (ELISA) in 30 heroin addicts as well as 24 normal control. Concentration differences between two groups were compared. Results: Lower levels of IL-2 and IFN-γ and higher levels of IL-4 and IL-6 were detected in the PMA and ionomycin stimulated PBMC culture supernatants of heroin addicts compared with control. Conclusion: Th1/Th2 functional imbalance exists in heroin addicts expressed as down-regulation of Th1 cells function, up-regulation of Th2 cells function. Th1/Th2 functional imbalance might explain the increased incidence of infection in heroin addicts.

关 键 词：TH1/TH2类细胞因子 海洛因依赖者 酶联免疫吸附试验(ELISA) 人外周血单个核细胞 IFN-Γ水平 细胞因子水平 细胞功能亢进 IL-4 IL-2 Th1细胞 Th2细胞 显著性差异 疾病发生率 PBMC 培养上清 体外刺激 IL-6 TL-6 对照比较 

分 类 号：R749.6[医药卫生—神经病学与精神病学]