山葡萄几丁质酶基因VCH3的克隆及植物表达载体的构建  

cDNA Cloning and Plant Expression Vector Construction of Vitis amurensis Chitinase Gene VCH3

作　　者：江源[1] 李海燕[1] 李大征 李艳杰 

机构地区：[1]吉林农业大学生物技术学院,长春130118 [2]白城市园林管理处,白城137000 [3]长春市南关区园林管理处,长春130022

出　　处：《吉林农业大学学报》2005年第3期272-275,共4页Journal of Jilin Agricultural University

基　　金：国家自然科学基金资助项目(30400300);吉林省科技发展计划项目(20030553-1;20040209-2);吉林农业大学博士基金资助项目

摘　　要：利用RT-PCR方法克隆了山葡萄几丁质酶基因VCH3的全长cDNA片段,并将该基因构建到双元载体pBI121质粒35S启动子下游,形成重组质粒pBIVCH3。通过冻融法将该重组质粒转化到根癌农杆菌LBA4404中,获得了植物表达载体。<Abstrcat> The full-length cDNA fragment of Vitis amurensis chitinase gene VCH3 was cloned by RT-PCR, then it was constructed to the downstream of 35S promoter in the binary vector pBI121, which formed the recombinant plasmid pBIVCH3. The plasmid pBIVCH3 was mobilized into Agrobacterium tumefaciens strain LBA4404 by freeze-melting methods, and a plant expression vector was thereby obtained.

关 键 词：山葡萄 几丁质酶基因VCH3 克隆 植物表达载体 

分 类 号：Q943[生物学—植物学] Q786