通过微量渗透泵应用肾上腺髓质素对大鼠低氧性肺动脉高压的调节作用  

Effect of Adrenomedullin Subcutaneously Administered by Mini-Osmotic Pump on Hypoxic Pulmonary Hypertension in Rats

作　　者：丁亚光[1] 齐建光[1] 杜军保[1] 唐朝枢[2] 

机构地区：[1]北京大学第一医院儿科,北京100034 [2]北京大学第一医院心血管病研究所,北京100034

出　　处：《实用儿科临床杂志》2005年第7期628-629,共2页Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金项目资助(30300376);国家重点基础研究发展规划项目资助(973G2000056905)

摘　　要：目的研究肾上腺髓质素(ADM)对大鼠低氧性肺动脉高压的调节作用。方法24只雄性Wistar大鼠随机分为对照组、低氧组、低氧+ADM组,每组各8只。对于低氧+ADM组大鼠通过微量渗透泵皮下持续给予ADM(300ng/h)。低氧2周后,以右心导管法测定肺动脉收缩压(sPAP)和肺动脉平均压(mPAP),测量体循环平均压(mSAP),检测右心室与左心室加室间隔比值[RV/(LV+S)]。结果低氧2周后大鼠sPAP、mPAP和RV/(LV+S)较对照组明显增高(P均<0.01),低氧+ADM组大鼠sPAP、mPAP和RV/(LV+S)较低氧组明显减低(P均<0.01),而mSAP在3组之间无明显差异。结论通过微量渗透泵皮下持续给予低氧大鼠ADM,缓解了低氧性肺动脉高压形成。Objective To study the modulation effect of adrenomedullin (ADM) on hypoxia pulmonary hypertension in rats.Methods Twenty-four male Wistar rats were randomly divided into control group ( n =8), hypoxic group ( n =8), hypoxic with ADM group ( n =8). ADM was subcutaneously administered into rats of hypoxic with ADM group by mini-osmotic pump (300 ng/h). After two weeks hypoxic challenge, systolic pulmonary arterial pressure (sPAP) and mean pulmonary arterial pressure (mPAP) were evaluated by a right cardiac catheterization procedure. Mean systemic artery pressure (mSAP) was measured. The ratio of right ventricular mass to left ventricular plus septal mass [RV/(LV+S)] was detected.Results sPAP, mPAP and RV/(LV+S) significantly increased in hypoxic rats compared with controls ( P <0.01, respectively). sPAP, mPAP and RV/(LV+S) significantly decreased in rats of hypoxic with ADM group compared with those of hypoxic group ( P <0.01, respectively). No significant difference was found in mSAP among the three groups.Conclusion ADM subcutaneously administered by mini-osmotic pump may alleviate the development of hypoxic pulmonary hypertension in rats.

关 键 词：肾上腺髓质素 低氧 肺动脉高压 微量渗透泵 

分 类 号：R543.2[医药卫生—心血管疾病]