乌苯美司对JAR,SKOV3和3AO细胞体外生长的抑制作用  

Inhibitory effect of ubenimex on human choriocarcinoma cell JAR and ovarian cancer cell SKOV3,3AO in vitro

作　　者：黄冬梅[1] 崔金全[1] 陶银贵[1] 

机构地区：[1]郑州大学第二附属医院妇产科,郑州450014

出　　处：《郑州大学学报（医学版）》2005年第4期641-644,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省教委自然科学基础研究项目98320008

摘　　要：目的:观察乌苯美司对人绒毛膜癌和卵巢癌细胞体外生长的抑制作用。方法:应用MTT比色法观察不同浓度的乌苯美司(0.5g/L、1g/L、5g/L、10g/L、15g/L、20g/L)对人绒毛膜癌JAR细胞、卵巢癌3AO细胞和SK-OV3细胞的生长抑制作用,溴乙啶和丫啶橙荧光染色荧光显微镜观察凋亡细胞DNA结构改变。结果:乌苯美司能抑制JAR细胞、3AO细胞和SKOV3细胞的生长,且对JAR和SKOV3细胞的抑制作用呈时效(r=0.412,r=0.339,P均=0.000)和量效(r=0.704,r=0.753,P均=0.000)关系。5g/L乌苯美司作用48h后荧光显微镜下见细胞染色质边聚,细胞核碎裂等凋亡改变。结论:乌苯美司通过细胞凋亡途径可抑制绒毛膜癌和卵巢癌细胞的生长。Aim: To study the inhibitory effect of ubenimex on human cellular lines of choriocarcinoma and ovarian cancer. Methods: JAR cellular line of choriocarcinoma and SKOV3 and 3AO cellular lines of ovarian cancer were treated with ubenimex at different concentrations(0.5 g/L,1 g/L,5 g/L,10 g/L,15 g/L,20 g/L), and the growth inhibition was analyzed by MTT assay. The changes of DNA structure were observed under fluorescence microscope with the fluorescence staining of ethidium bromide. Results: Ubenimex inhibited the proliferation of JAR and SKOV3 cells in vitro in concentration- (r=0.704,r=0.753,P=0.000) and time- (r=0.412,r=0.339,P=0.000) dependent manners,respectively.After being treated by ubenimex at 5 g/L, the chromatin was located beside the membrane of cellular nucleus, or smashed like the changes of cellular apoptosis. Conclusion: Ubenimex inbibits the growth of human cell lines of choriocarcinoma and ovarian cancer through inducing cell apoptosis.

关 键 词：乌苯美司 凋亡 JAR细胞 SKOV3细胞 3AO细胞 

分 类 号：R737.3[医药卫生—肿瘤]