胃癌组织CD1α和survivin表达及临床意义  

Clinical significance of CD1a and survivin expression in gastric carcinoma

作　　者：郭建巍[1] 秦力维[2] 吕同德[1] 杨霄鹏[1] 刘斌[3] 昌业伟[1] 

机构地区：[1]兰州军区兰州总医院实验科,甘肃省兰州市730050 [2]兰州军区兰州总医院眼科,甘肃省兰州市730050 [3]兰州军区兰州总医院病理科,甘肃省兰州市730050

出　　处：《世界华人消化杂志》2005年第12期1425-1428,共4页World Chinese Journal of Digestology

摘　　要：目的:观察胃癌组织中树突状细胞及树突状细胞前体的分布,并探讨survivin作为胃癌生物治疗靶抗原的可能性.方法:用免疫组织化学染色对不同类型胃癌组织132例(中分化腺癌72例,低分化腺癌60例)CD1α,CD68,S100和survivin蛋白的表达进行了检测.结果:中分化腺癌CD1α表达率为33%(24/72),低分化腺癌CD1α表达率为10%(6/60),二者有显著性差异(X2=6.56,P<0.05).中分化腺癌$100表达率为50%(36/72),低分化腺癌表达率30%(18/60),二者无显著性区别(P>0.05).CD1α阳性胃癌组织S100表达均阳性,二者有良好的一致性.中分化腺癌CD68表达为91.6%(66/72),低分化腺癌表达率为60%(36/60),二者无显著性差别(P>0.05),CD68和CD1α在中分化腺癌和低分化腺癌中的分布也有良好的一致性.Survivin在中分化腺癌的表达为91.7%(66/72),低分化腺癌表达为90%(54/60),二者无显著性差别(P>0.05).结论:survivin和DC的结合在胃癌生物治疗中具有潜在而重要的应用价值,以survivin为靶抗原的胃癌个体化DC疫苗值得进一步深入研究.AIM: To investigate the distribution of dendritic cells (DCs) and DC precursors in gastric carcinoma (GC) and to discuss the possibility of survivin as the target antigen in GC immunotherapy. METHODS: Immunohistochemical staining was used to detect the expression of CD1 α, CD68, S100 and survivin protein in 132 cases of GC tissues, including 72 cases of moderately differentiated adenocarcinomas and 60 poorly differentiated ones. RESULTS: The expression of CD1 α in moderately differentiated GCs(33%, 24/72) was significantly higher (X2 = 6.56, P<0.05) than that in poorly differentiated ones (10%, 6/60). S100 expression was not markedly different between moderately (50%, 36/72) and poorly (30%, 18/60) differentiated GCs (P>0.05). The expression of CD1α was positively related to S100 protein. The distribution of CD68 molecules in moderately and poorly differentiated GCs was and 60% respectively and there was no significantly difference between them (P>0.05). The expression CD1α was also positively related to CD68 in GC.The expression of survivin protein was 91.7% (66/72) and 90%(54/60) in moderately and poorly differentiated GCs respectively, and there was no significant difference between them. CONCLUSION: Combination of survivin and DC may play a valuable role in GC immunotherapy, and further studies on individualized DC vaccine against GC targeted on survivin antigen should be made in the future.

关 键 词：胃癌组织 CD1α SURVIVIN 表达 树突状细胞前体 树突状细胞 

分 类 号：R735.2[医药卫生—肿瘤]