Nogo-66受体mRNA在成年大鼠视神经中的表达及意义  

Expression and significance of NgR mRNA in adult rats′ optic nerve

作　　者：陈春林[1] 叶剑[1] 张巍[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所眼科,重庆400042

出　　处：《中华眼底病杂志》2005年第4期246-248,共3页Chinese Journal of Ocular Fundus Diseases

基　　金：国家自然科学基金资助项目(30270458);全军十五课题项目(01MA175)

摘　　要：目的观察Nogo-66受体(NgR)mRNA在成年大鼠视神经的表达并探讨其意义。方法取8只成年大鼠视神经及坐骨神经,将拟杂交的组织切片分为3组:视神经实验组、坐骨神经对照组、视神经阴性对照组。采用原位杂交技术观察NgRmRNA在视神经和坐骨神经中的表达。结果大鼠视神经切片中NgRmRNA均表达阳性,阳性信号沿视神经长轴排列;所有大鼠坐骨神经切片中NgRmRNA的表达均为阴性。结论在成年大鼠视神经中NgRmRNA广泛表达,而坐骨神经中不表达,提示NgR阳性表达及分布与视神经再生能力低下密切相关。Objective To observe and evaluate the expression and significance of Nogo-66 receptor (NgR) mRNA in adult rats′ optic nerve. Methods Optic and sciatic nerves of 8 adult rats were used to make the sections, which were divided into 3 groups: optic-nerve experimental group, sciatic-nerve control group, and optic-nerve negative control group. In situ hybridization was used to observe the expression of NgR mRNA in optic nerve and sciatic nerve. Results The expression of NgR mRNA in the 8 rats was positive in optic nerve and negative in sciatic nerve. The positive signals were arranged along the long axis of optic nerve. Conclusion The expression of NgR mRNA is positive in optic nerve while negative in sciatic nerve in adult rats, which suggests that the positive expression and distribution of NgR may be related to the poor regenerate ability of optic nerves.

关 键 词：视神经 免疫学 坐骨神经 神经再生 基因表达 Nogo-66受体 mRNA 大鼠 坐骨神经 

分 类 号：R774.6[医药卫生—眼科] R446.9[医药卫生—临床医学]