幽门螺杆菌改变人肝细胞系HepG2蛋白质表达谱  

Helicobacter pylori changes the protein expression profile of HepG2

作　　者：张艳[1] 范学工[1] 陈仁[1] 肖志强[2] 冯雪萍[2] 田雪飞[1] 陈朝晖[2] 

机构地区：[1]中南大学湘雅医院传染科 [2]中南大学湘雅医院医学实验研究中心卫生部肿瘤蛋白质组学重点实验室,湖南长沙410008

出　　处：《中国人兽共患病杂志》2005年第7期561-565,共5页Chinese Journal of Zoonoses

摘　　要：目的研究H.pylori作用后肝细胞系HepG2蛋白质表达谱的改变,通过对差异蛋白质点的鉴定与分析,以探讨H.pylori对肝细胞的病理作用。方法采用体外肝细胞和H.pylori共培养,应用双向凝胶电泳分离H.pylori处理前后细胞总蛋白,筛选出差异表达的蛋白质点,并进行质谱分析鉴定。结果鉴定出7种表达上调的蛋白质点,包括整合素-β1、蛋白激酶C-α、LIM同源盒蛋白Lhx1、真核翻译起始因子2-β亚单位、PINCH蛋白、MAPK激酶3、Ras相关蛋白Rab-37等。这些蛋白质参与了基因表达调控、细胞免疫、细胞生长、信号传导等众多事件。结论H.pylori可能通过介导这些蛋白质的上调而发挥对HepG2细胞的病理作用。这种蛋白质组的差异分析有助于进一步研究H.pylori与人类肝胆疾病的关系。To explore the pathological effect of H. pylori on human hepatic cells, this study was conducted to investigate the change of the protein expression of HepG2 caused by H. pylori treatment, and to identify differential expression proteins. H. pylori was co-cultured with HepG2 for 6h, two-dimensional gel eletrophoresis (2-DE) was used to gain the protein pattern of HepG2 cells before and after treated with H. pylori. The differential expression proteins were identified by MALDI-TOF-MS. Seven proteins which were up-regulated in H. pylori treated HepG2 were identified. These proteins included integrin beta-1, protein kinase C alpha, LIM/homeobox protein Lhx1, eIF-2-beta, MAP kinase kinase 3, PINCH protein and Ras-related protein Rab-37, which involved in transcription regulation, metabolism, signaling pathway and so on. H. pylori may exert the pathological effect on HepG2 cells by up-regulating the expression of proteins. It suggests that the differential expression analysis of proteomes be useful to further study of the relation of H. pylori and human hepatobiliary diseases.

关 键 词：细胞系HepG2 蛋白质表达谱 幽门螺杆菌 H.pylori 蛋白激酶C-Α 人肝 整合素β-1 MAPK激酶 基因表达调控 病理作用 s相关蛋白 差异表达 分析鉴定 表达上调 结果鉴定 电泳分离 Β亚单位 起始因子 细胞免疫 细胞生长 信号传导 

分 类 号：R977.11[医药卫生—药品] R392.3[医药卫生—药学]