APA-BCC镇痛微囊在癌痛患者脑脊液中的生物学变化  

Biological Changes of APA-BCC Analgesic Microcapsule in Cerebrospinal Fluid of Patients with Carcinomatous Pain

作　　者：罗芸[1] 李雁凌[1] 薛毅珑[1] 郭水龙[1] 高宇红[1] 崔忻[1] 

机构地区：[1]解放军总医院老年医学研究所细胞生物学研究室,北京100853

出　　处：《标记免疫分析与临床》2005年第2期97-99,共3页Labeled Immunoassays and Clinical Medicine

基　　金：国家高科技研究发展计划(八六三)课题(2002AA216141);军队"十五"重点项目资助课题(01Z031)

摘　　要：了解海藻酸钙多聚赖氨酸海藻酸钙微囊化牛肾上腺嗜铬细胞(APABCC微囊)植入癌痛患者脑脊液中的形态、活率及亮氨酸脑啡肽(LEK)分泌的变化。将APABCC微囊按常规腰穿方法植入癌痛患者蛛网膜下腔。7或8天时采取脑脊液,观察APABCC的形态、细胞活率,用放射免疫法测定脑脊液中LEK的含量。移植7天后,患者视觉模拟疼痛评分(VAS)均值由移植前的8.8降为2.4;脑脊液中APABCC微囊形态无明显变化;细胞活率由平均91.2%降为89.1%;LEK含量较移植前增加了1.65倍。将APABCC微囊植入癌痛病人脑脊液中能够保持细胞存活、分泌亮啡肽,并产生镇痛效应。To explore the changes of alginate-polylysine-alginate microcapsulated bovine adrenal medullary chromaffin cells (APA-BCC microcapsules) in morphology, survival rate and leucine-enkephalin secretion after they were transplanted into CSF of cancer-pain patients, the APA-BCC microcapsules were Implanted into cavitas subarachnoidealis of cancer-pain patients by conventional lumbar puncture. After 7 or 8 days, cerebrospinal fluid was collected and the morphology of the APA-BCC microcapsule, the survival rate of cells were observed and secretory volume of leucine-enkephalin was assayed by radioimmunity method. Seven days after transplantation, the mean VAS decreased from 8.8 to 2.4, the survival rate of cells averagely reduced from 91.2% to 89.1%, morphology of APA-BCC microcapsules did not change obviously and secretory volume of leucine-enkephalin went up 1.65 times compared with that at pretransplantation. In conclusion, APA-BCC can survive, secret leucine-enkephalin and produce analgesic effect after transplanted into CSF of cancer-patients.

关 键 词：微囊化细胞 移植手术 细胞活率 亮氨酸脑啡肽 脑脊液 APA-BCC 镇痛微囊 癌痛 脑脊液 生物学 

分 类 号：R730.5[医药卫生—肿瘤]