大鼠死亡后外周血和尿液中心脏型脂肪酸结合蛋白的研究  

Study on the postmortem change of plasma and urine concentrations of H-FABP in rat

作　　者：郎博娟[1] 孟祥志[1] 王琼[1] 

机构地区：[1]武汉大学医学院法医教研室,湖北武汉430071

出　　处：《医学研究生学报》2005年第7期599-600,608,共3页Journal of Medical Postgraduates

基　　金：湖北省卫生厅科研基金资助项目(批准号:JX1B009)

摘　　要：目的:观察心肌梗死大鼠死亡后不同时间段外周血和尿液中心脏型脂肪酸结合蛋白(H FABP)含量变化。方法:按Johns法结扎大鼠左冠状动脉前降支,复制心肌梗死模型,用酶联免疫吸附(ELISA)法测定大鼠死亡后不同时间段外周血和尿液中H FABP含量。结果:①实验组外周血H FABP浓度显著高于对照组;随着死亡时间的延长,其含量持续升高,12h组H FABP含量显著高于0h组,48h组显著高于24h组。②实验组尿液H FABP浓度均显著高于对照组,大鼠死亡后其浓度呈现波浪状升高;心肌梗死大鼠死亡6h组尿液H FABP浓度显著高于0h组,12、24h时H FABP含量降低,但仍显著高于0h组,48h组尿液H FABP浓度再次升高,显著高于6h组。结论:心肌梗死大鼠死亡后外周血和尿液中H FABP浓度受组织自溶的影响出现异常升高,因而H FABP外周血和尿液浓度检测用于死后诊断心肌梗死性猝死证据不足。Objective: To observe the changes of heart-type fatty acid binding-protein(H-FABP) in the femoral vein(periphe ral blood) and urine of rats with myocardial infarction in different postmortem intervals. Methods: Acute myocardial infarction (AMI) was duplicated by ligating the anterior branch of the left coronary artery following the method of Johns in rats. Concentrations of H-FABP were detected with enzyme-linked immuno-sorbent assay (ELISA). Results: The concentrations of H-FABP in experiment group were obviously higher than that in control group. Along with the time, the concentrations of H-FABP in experiment group rose consistently, the concentration of H-FABP in 12 h group rose markedly, and it was obviously higher than in 48h that in 24h. The H-FABP concentrations in urine of the experiment group were obviouslyhigher than the one in control group. The urine concentrations of H-FABP in experiment group increased by wavy in postmortem intervals, the concentration of H-FABP in (6h) group was obviouslyhigher than that in 0h group, 12h and 24h postmortem, the H-FABP concentrations decreased, but still higher than that of 0h group and 48h group, the concentration of H-FABP rose markedly and washigher than 6h group. Conclusion: Affected by autolysis, the H-FABP concentrations of plasma and urine appeared abnormal raise and can not be used in postmortem diagnosis of AMI.

关 键 词：急性心肌梗死 心脏型脂肪酸结合蛋白 酶联免疫吸附实验 自溶 

分 类 号：Q513[生物学—生物化学]