矩类迁移比作为单细胞凝胶电泳指标的研究  

Study on ratio of moment move and ratio of inertia move as indices of single-cell gel electrophoresis

作　　者：朱志良[1] 庄志雄[2] 黎建明[1] 吴礼康[1] 肖惠贞[1] 

机构地区：[1]深圳市宝安区疾病预防控制中心,广东深圳518101 [2]深圳市疾病预防控制中心

出　　处：《毒理学杂志》2005年第2期117-119,共3页Journal of Toxicology

基　　金：国家自然科学基金资助项目 (39970 636);深圳市科技立项项目 (2 0 0 4 0 4 1 97)

摘　　要：目的　研究单细胞凝胶电泳新指标。方法　采用H2 O2 对人外周淋巴细胞急性染毒作为DNA损伤模型,应用新型图像分析系统分析矩迁移比与惯量迁移比等指标。结果　Olive尾矩、矩迁移比和惯量迁移比3个指标与H2 O2剂量之间存在良好的线性剂量 效应关系,而尾长指标则在H2 O2 的高剂量范围(>160 μmol L)内出现饱和平台现象,且显示矩迁移比和惯量迁移比的变异系数较Olive尾矩有较小。结论　矩迁移比和惯量迁移比既有矩类指标的优点,又有比值类指标的优点,值得推广应用。Objective To study new indices of single-cell electrophoresis(SCGE):ratio of moment move (R_M) and ratio of inertia move (R_I).Method Using human lymphocyte treated by model DNA breaking agent, H_2O_2,several important analytical parameters were observed such as tail length (TL), tail moment (TM), tail inertia (TI), tail DNA %, R_M,R_I.Results It showed that TM,R_M and R_I appeared to have a good linear response relationship with H_2O_2 damage, but TL had such a linear relationship above 160 μmol/L.Conclusion Two new highly effective indices for SCGE, namely, R_M and R_I are two good indices of SCGE examination.

关 键 词：单细胞凝胶电泳 迁移 指标 剂量-效应关系 OLIVE 外周淋巴细胞 H2O 损伤模型 急性染毒 系统分析 图像分析 剂量范围 变异系数 推广应用 DNA 

分 类 号：R994.6[医药卫生—毒理学] R730.55[医药卫生—药学]