能量可控陡脉冲损伤兔肝脏实验性肿瘤的病理学研究  

Pathological studies of tissue damage in experimental rabbit liver cancer induced by energy controllable steep pulses

作　　者：王萍玲[1] 胡丽娜[1] 杨孝军[1] 李均[1] 孙才新[2] 熊兰[2] 姚陈果[2] 王士彬[2] 

机构地区：[1]重庆医科大学附属第二医院妇产科,400010 [2]重庆大学高电压与电工新技术教育部重点实验室

出　　处：《中华肝脏病杂志》2005年第7期516-519,共4页Chinese Journal of Hepatology

基　　金：国家自然科学基金(30371619)

摘　　要：目的评估陡脉冲治疗种植性兔肝癌VX2肿瘤的有效性。方法30只建模成功的新西兰大白兔分别分成实验组和对照组,其中15只兔肝肿瘤接受陡脉冲处理,其余作为对照组,处理前后皆行光、电镜观察肿瘤组织的病理学改变,纪录各荷瘤兔的生存时间和转归。结果陡脉冲对兔肝肿瘤组织有明显的破坏作用,脉冲处理后的坏死肿瘤与正常组织界限清晰,超微结构显示出不可逆性的细胞改变;经陡脉冲治疗后的荷瘤兔生存期明显延长,治疗组和未治疗组荷瘤兔的平均生存时间分别为83.1d和39.0 d,Z=-2.943, P<0.0 1。结论陡脉冲能有效治疗兔实验性VX2肝癌,对靶区周围正常组织是安全的,陡脉冲有望成为肿瘤综合治疗中有效的替代疗法。Objective To evaluate the efficacy of energy controllable steep pulses (ECSP) in the treatment of rabbit VX2 cancer implanted in livers. Method A tumor model was successfully established using 30 rabbits. ECSP were applied to liver cancer in half of these rabbits and the rest were used as controls. After exposure to ECSP, tissues were obtained and subjected by routine HE and transmission electron microscopic (TEM) observation. The survival time of the animals and the statuses of each group were recorded. Results From pathological observations, ECSP showed effectively destructive action compared with that of the unexposed group. A clear borderline can be seen between necrotic cancer and its surrounding normal tissue. Irreversible cell changes were present under TEM. The survival periods of the experimental and control group were 83.1 days and 39.0 days respectively, and there was a significant difference between the two groups (Z = -2.943, P < 0.01). Conclusion ECSP can effectively treat rabbit VX2 cancer implanted in the liver; also it is safe for its surrounding normal tissues. ECSP can be a useful method for local treatment of liver cancer.

关 键 词：能量可控 陡脉冲损伤 兔 肝脏 实验性肿瘤 病理学 替代疗法 

分 类 号：R735.7[医药卫生—肿瘤]