大鼠实验性肝硬化门脉高压形成过程中内皮素及其转化酶的动态变化  

Dynamic changes of endothelin-1 and ECE during the process of experimental cirrhotic portal hypertension in rats

作　　者：许夕海[1] 陈澍[2] 卢清[2] 施光峰[2] 

机构地区：[1]安徽医科大学附属医院感染科,合肥230022 [2]复旦大学附属华山医院

出　　处：《肝脏》2005年第2期89-91,共3页Chinese Hepatology

摘　　要：目的观察大鼠实验性肝硬化门脉高压形成过程中,外周血内皮素(ET1)含量和肝脏前内皮素原(ppET1)和内皮素转化酶(ECE)mRNA表达的动态变化,以期对其在门脉高压中动态变化的特点有进一步的了解。方法通过四氯化碳(CCl4)介导大鼠实验性肝硬化门脉高压模型,放免法检测ET1,半定量RTPCR法观察不同时期肝组织中ppET1和ECEmRNA的表达差异。结果与对照组相比,模型组中外周血ET1含量和肝脏ppET1的表达分别从第8周和第6周开始显著升高,且ET1的升高与门脉高压形成呈显著相关,而肝脏中ECE的表达无明显差异。结论外周血中ET1含量的增高和肝脏中ppET1表达的增加可能是形成肝硬化门脉高压的重要原因之一。Objective To further evaluate the content of bloocl endothelin(ET-1), the expression of preproendothelin (ppET-1) and endothelin coverting enzyme(ECE) mRNA in the liver during the process of cirrhotic portal hypertension(CPH).Methods CPH rats were induced by CCl 4. The concentrations of ET-1 in plasma were studied by radioimmunoassay and the hepatic differences of gene expressions of ppET-1 and ECE were performed by semi-quantitative reverse transcription polymerase chain reaction CPH.Results The ET-1 concentration of the CPH group increased by 59% than that of the control group in the 8th week and raised much significantly in the 10th week. There was a positive correlation(r=0.79,P<0.01) between the protal pressure(PP) and the plasma ET-1 concentration. The hepatic gene expression of ppET-1 was upregulated by 46% of the CPH group than that of the control group in the 6th week and no significant difference of hepatic ECE expression between the two groups during the whole development of CPH. Conclusion The increase of ET-1 concentration and the up-regulation of hepatic ppET-1 expression maybe play an important role in the pathogenesis of CPH.

关 键 词：大鼠 实验性肝硬化 门脉高压 内皮素 转化酶 

分 类 号：R575.2[医药卫生—消化系统] R657.34[医药卫生—内科学]