视网膜母细胞瘤易感基因（Rb基因）抑制人肝癌细胞的生长  

SUPPRESSION OF HUMAN HEPATOCELLULAR CARCINOMA CELL GROWTH OF RETINOBLASTOMA SUSCEPTIBILITY GENG

作　　者：陈大年[1] 李岱宗[1] 刘超亭 姚明[1] 李洪年[1] 蒋惠秋[1] 李安仁[1] 黄倩[1] 顾健人[1] 罗成仁[1] 

机构地区：[1]华西医科大学附属第一医院眼科,上海市肿瘤研究所生化室

出　　处：《中华眼底病杂志》1994年第1期21-24,T004,共5页Chinese Journal of Ocular Fundus Diseases

基　　金：国家自然科学基金

摘　　要：利用流式细胞学检查及Ｓｏｕｔｈｅｒｎ、Ｗｅｓｔｅｒｎ杂交技术对中国人肝癌细胞ＳＭＭＣ７７２１进行分析，发现该细胞ＤＮＡ指数为０．８ｌ，为亚倍体细胞。该细胞Ｒｂ基因的５′端存在内部缺失，Ｒｂ基因失活，没有Ｒｂ蛋白产生。将正常人ＲｂｃＤＮＡ正向插入逆转录病毒载体ＤＯＬ，得到重组逆转录病毒载体ＤＯＬＲＢ。用电穿孔转染技术把ＤＯＬＲＢ转入ＳＭＭＣ７７２１细胞，发现Ｒｂ基因导致约７５％的肝癌细胞死亡，另外２５％的肝癌细胞则因外源性Ｒｂ基因突变失活而继续存活。he Chinese human hepetocellular carcinoma cell SMMC772l has been analysed by flowcytometry, Southern blotting and Western blotting,The results indicated that SMMC772l cell is ahguploid cell with a 0.81 DNA index,and that SMMC772l cell has internal deletion in the 5′-end ofits Rb gene and has no Rb gene product(Rb protein).The normal Rb cDNA has ben inserted into aretrovirus vector DOL and intreduced into SMMC772l cells by electrporation transfection technique。About 75%of transfected SMMC772l cells have been killed by Rb gene product. The remain 25%cells are alive as exogenous Rb gene has been mutationally inactivated。

关 键 词：视网膜 母细胞瘤 RB基因 肝癌细胞 

分 类 号：R730.231.3[医药卫生—肿瘤]