伪狂犬病病毒Bartha-K61株UL49基因的克隆和序列分析  

Cloning and sequencing of the UL49 gene of pseudorabies virus Bartha-K61 strain

作　　者：刘建华[1] 李娜[1] 仇华吉[1] 田志军[1] 童光志[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室

出　　处：《中国预防兽医学报》2005年第4期269-272,共4页Chinese Journal of Preventive Veterinary Medicine

摘　　要：根据已经发表的伪狂犬病病毒(PRV)国内Ea株UL49基因序列,设计并合成了1对引物,通过PCR方法扩增到了PRVBartha-K61株UL49基因的编码区,并克隆到pMD18_T载体中。重组质粒pMD_UL49经XhoI酶切和PCR鉴定后,进行了序列测定和分析。结果表明,重组质粒pMD_UL49含有PRVBartha_K61株UL49基因的编码区。同源性分析显示,PRVBartha_K61株UL49基因序列与国外Kaplan株、国内Ea株相应基因的核苷酸同源性分别为98.9%和94.1%,推导的氨基酸序列同源性分别为96.7%和87.2%。According to the published sequence of UL49 gene of pseudorabies virus ( PRV) Ea strain,a pair of primers were designed to amplify the UL49 gene of pseudorabies virus (PRV) Bartha_K61 strain.The UL49 gene was amplified by polymerase chain reaction (PCR),subsequently cloned into pMD18_T vector.The recombinant plasmid pMD_UL49 was identified by PCR and Xho I restrictive digestion,then sequenced. The seqencing result showed that the recombinant plasmid pMD_UL49 contained the UL49 gene of 750 bp.The nucleotide homology of UL49 gene between Bartha_K61 and Kaplan and Ea strain was 98.9 % and 94.1 %,respectively.The deduced amino acid homology of UL49 gene was 96.7 % and 87.2%,respectively.

关 键 词：伪狂犬病病毒 UL49基因 克隆 序列分析 

分 类 号：S852.65[农业科学—基础兽医学] Q785[农业科学—兽医学]