甘蓝型油菜黄籽品种SSR指纹图谱的构建  

SSR FINGERPRINTING OF YELLOW-SEEDED RAPESEED VARIETIES (BRASSICA NAPUS L.)

作　　者：雷天刚[1] 张学昆[1] 陆合[1] 李加纳[1] 徐新福[1] 范其新[1] 谌利[1] 

机构地区：[1]西南农业大学农学与生命科学学院,重庆400716

出　　处：《西南农业大学学报（自然科学版）》2005年第3期361-364,共4页Journal of Southwest Agricultural University

基　　金：863资助项目"优质高产农作物新品种培育"(2003AA207150)

摘　　要：采用SSR标记技术构建5个甘蓝型油菜黄籽品种的指纹图谱,用于鉴别种子真伪。以19个甘蓝型油菜黄籽品种(系)为材料,从100对SSR引物中筛选出2对多态性最高的SSR引物进行PCR扩增。经非变性聚丙烯酰胺凝胶电泳检测显示,5个甘蓝型油菜黄籽品种都存在特异性带,成功构建出甘蓝型油菜黄籽品种的指纹图谱。表明SSR标记技术是种质鉴定的一种高效方法。Fingerprinting of 5 yellow-seeded rapeseed varieties (Brassica napus L.) of China was constructed using the SSR technique so as to distinguish false seeds from true seeds. Nineteen yellow-seeded rapeseed varieties and lines were studied in this experiment. Two primer pairs selected from 100 showed ample polymorphism and could distinguish all the materials studied; and they were used for PCR. Specific bands were detected in 5 varieties in un-denaturalized PAGE, and thus the DNA fingerprinting of the 5 varieties was constructed successfully. The results indicate that SSR marker technique is highly efficient for authentication of plant materials.

关 键 词：甘蓝型黄籽油菜 SSR 指纹图谱 

分 类 号：S565.401[农业科学—作物学]