8-Br-cAMP对膀胱癌细胞中c-erbB2c-myc表达的影响  

Effects of 8-Br-cAMP on the Expression of c-erbB-2 c-myc Gene in Bladder Carcinoma Cells

作　　者：单中杰[1] 侯箐岚 陈奎生[2] 杨庆祥[1] 刘慧明[1] 翟晓磊[1] 葛雷[1] 周俊英[1] 

机构地区：[1]郑州市第五人民医院,郑州市450003 [2]郑州大学一附院病理科,郑州市450052

出　　处：《医药论坛杂志》2005年第13期7-8,10,共3页Journal of Medical Forum

基　　金：河南省科委科技攻关计划项目(0324410004)

摘　　要：目的探讨环腺苷酸(cAMP)类似物8-溴-环腺苷酸(8-Br-cAMP)对人原代培养的膀胱癌细胞中c-erbB-2、c-myc蛋白及mRNA表达的影响。方法原代培养膀胱癌细胞,采用完整细胞免疫组化、原位杂交方法检测膀胱癌细胞中c-erbB-2、c-myc蛋白及mRNA的表达。结果8-Br-cAMP处理后的膀胱癌细胞中c-erbB-2、c-myc蛋白及mRNA表达水平明显低于未处理组,两组相比差异均有统计学意义(P<0.05)。结论8-Br-cAMP可抑制膀胱癌细胞中c-erbB-2、c-myc蛋白及mRNA表达,进而抑制肿瘤细胞的分裂和增殖。Objective To study the effects of 8-Br-cAMP on the expression of c-erbB-2,c-myc gene and mRNA in bladder carcinoma cells. Methods After human bladder carcinoma cells were cultured primarily in the media containing 10-5mol/L of 8-Br-cAMP ,the alteration of expression of c-erbB-2,c-myc gene and mRNA were observed by using immunohistochemistry SP method and in situ hybridization. Results The expression of c-erbB-2,c-myc mRNA and protein in experimental group were weaker than that in control group , there was significant difference between control group and experimental group (P<0.05). Conclusion 8-Br-cAMP could inhibited the expression of c-erbB-2,c-myc and mRNA in bladder carcinoma cells, it indicated that 8-Br-cAMP was an effective inhibitor for mitotic and proliferation of tumor cells.

关 键 词：8-溴-环腺苷酸膀胱癌细胞c-erbB-2 c—myc 

分 类 号：R737.14[医药卫生—肿瘤]