精液白细胞含量与精子顶体酶活性分析  

Semen White Blood Cells Volumetric Count and Sperm Acrosin Activity

作　　者：楚雅玲[1] 岳林[2] 

机构地区：[1]河南科技大学第一附属医院检验科,洛阳市471003 [2]河南省洛阳市中心医院生殖医学中心,洛阳市471009

出　　处：《医药论坛杂志》2005年第13期38-39,共2页Journal of Medical Forum

摘　　要：目的研究精液白细胞含量对精子顶体酶活性的影响。方法采用正甲苯胺蓝过氧化物酶法对精液中白细胞进行定量测定并与精子顶体酶活性进行相关性分析。结果383例男性不育就诊者中106例精液白细胞>1×106个/ml,设为阳性组;277例精液白细胞≤1×106个/ml,设为阴性组:阴性组精子顶体酶活性(22.21±10.25)uIU/106精子,明显高于阳性组精子顶体酶活性(12.81±9.23)uIU/106精子,两组有显著性差异(P<0.001)。结论精液白细胞含量增高可影响精子顶体酶活性。Objective To study the relationship between semen white blood cells volumetric count and sperm acrosin activity.Method Staining for volumetric count of leukocytes in semen and to carry out correlation analysis with sperm acrosin activity.Results Semen WBC>1×106/ml were 106 cases in 383 male infertility,set up into positive group;Semen WBC≤1×106/ml were 277 cases,set up into negative group; Sperm actosin activity in negative group was (22.21±10.25)uIU/106sperm and it was observed to be higher evidently compared to positive group(12.81±9.23)uIU/106sperm.Consequently,there was great difference between positive group and negative group(p<0.001).Conclusion Sperm WBC have effect on sperm acrosin activity.

关 键 词：细胞含量 酶活性分析 精子顶体酶活性 正甲苯胺蓝过氧化物酶法 精液白细胞 相关性分析 显著性差异 定量测定 男性不育 就诊者 ML 阳性 阴性 

分 类 号：R457.7[医药卫生—治疗学] R512.62[医药卫生—临床医学]