肝癌特异性AFP亚型分离检测法的建立  

Detection of hepatoma-specific alpha-fetoprotein isoform

作　　者：肖明兵[1] 倪润洲[1] 金飞[1] 黄介飞[1] 孟宪镛[1] 

机构地区：[1]南通大学附属医院消化病研究室,江苏南通226001

出　　处：《临床检验杂志》2005年第4期247-250,共4页Chinese Journal of Clinical Laboratory Science

基　　金：江苏省社会发展科技项目(BS2004528)。

摘　　要：目的建立聚丙烯酰胺凝胶电泳(PAGE)结合蛋白印迹(WB)技术分离检测肝癌特异性甲胎蛋白亚型(HS-AFP)方法。方法以自然阶段梯度PAGE分离血清甲胎蛋白(AFP),WB技术检测AFP亚型,摸索研究AFP分离、转膜、呈色检测的实验条件。对82例肝癌、95例良性肝病患者及44例健康献血员的血清AFP亚型进行分离检测,分析肝癌与良性肝病AFP亚型谱型的差别。结果成功建立了血清AFP亚型分离检测法,分离检测出HS-AFP。肝癌患者HS-AFP阳性率为74.4%,显著高于良性肝病组。结论建立的HS-AFP分离检测法有助于肝癌诊断。Objective To establish a method based on polyacrilamide gel electrophoresis (PAGE) and Western blot for detection of hepatoma-specific alpha-fetoprotein (HS-AFP) isoform.Methods Serum AFP was separated by native polyacrylamide gel electrophoresis,and AFP isoforms were subsequently detected by Western blot.The experimental conditions for separation,transfer and staining of AFP isforms were optimized.AFP isoforms in serum samples from 82 cases of primary hepatic cancer and 139 cases of various benign liver diseases were detected.The difference of electrophoretic patterns for AFP isoforms between primary hepatic cancer and benign liver diseases were analyzed.Results HS-AFP in serum was distinctly separated and a method for detection of HS-AFP isoforms was successfully established in this study.The results of detection for clinical samples showed that the positive rate for HS-AFP in the patients with primary hepatic cancer was 74.4%, which was significantly higher than that in benign liver diseases ( P <0.01).Conclusion The method established in this study for detection of HS-AFP in this study is helpful for the diagnosis of primary hepatic cancer.

关 键 词：肝癌特异性 检测法 聚丙烯酰胺凝胶电泳 血清AFP 分离检测 血清甲胎蛋白 良性肝病 健康献血员 蛋白亚型 蛋白印迹 PAGE 技术检测 实验条件 肝病患者 肝癌患者 肝癌诊断 HS 阳性率 

分 类 号：R575[医药卫生—消化系统] R446.62[医药卫生—内科学]