烟草粉螟高毒力Bt菌株的发酵条件  

Fermentation Conditions for High Toxicity Bt Isolates against Ephesia elutella Hübner

作　　者：高家合[1] 

机构地区：[1]云南省烟草科学研究所,云南省玉溪市聂耳路47号653100

出　　处：《烟草科技》2005年第7期38-40,共3页Tobacco Science & Technology

基　　金：云南省科技厅"十五"攻关项目(2001NG08);云南省烟草公司资助项目(01A06)。

摘　　要：对烟草粉螟有较高毒力的苏云金杆菌Bt33、Bt53、BtHB3株菌株的发酵培养条件进行了试验。结果表明:3株菌株的最佳培养温度为30℃、pH值为7.5、转速为180rpm,Bt33、Bt53的最佳培养时间为40h,BtHB为36h,BtHB培养的最佳空气量为19/20[(培养容器容积-培养基体积)/培养容器容积],Bt33、Bt53均为9/10。The fermentation conditions of Bt33、Bt53、BtHB isolates of Bacillus thuringiensis, which were high toxicity to Lepidoptera insect, were studied. The results indicated that the optimum culture conditions for all tested isolates were: 30℃, pH 7.5, rotating speed 180 rpm. The optimum culture time for Bt33 and Bt53 isolates was 40 hours, while that for BtHB was 36 hours. And the optimum air volume for Bt33 and Bt53 isolates was 9/10 (container volume-medium volume/container volume), while that forBtHB was 19/20.

关 键 词：烟草粉螟 苏云金杆菌 发酵 

分 类 号：S476.8[农业科学—农业昆虫与害虫防治]