BTG2基因在大鼠胚胎胰腺不同发育阶段的表达  

The Expression Profile of BTG2 in Different Development Stages of Rat Embryonic Pancreas

作　　者：胡静静[1] 滕丽萍[1] 袁庆新[1] 袁栎[1] 周锦勇[1] 仲燕[1] 刘莉洁[1] 德伟[1] 

机构地区：[1]南京医科大学生物化学系,南京210029

出　　处：《中国生物工程杂志》2005年第7期71-75,共5页China Biotechnology

基　　金：江苏省科委应用基础资助项目(BK2001119)

摘　　要：利用高密度寡核苷酸芯片技术对大鼠胚胎胰腺发育中晚期(E12.5,E18.5,E15.5)调节内外分泌部细胞发育分化及功能代谢的基因的表达趋势进行研究。并用RT-PCR进行验证。用获得的基因信息对:NCBI等公共数据库进行检索,结果发现对细胞的分化、增殖和凋亡起调节作用的BTG2基因在大鼠胚胎胰腺E12.5、E15.5、E18.5天及成年、新生大鼠胰腺中均有表达,且E18.5天的表达量高于其他时期5倍多。推测BTG2可能在大鼠胚胎胰腺内外分泌细胞分化发育的不同阶段起到了促进作用,并参与胚胎胰腺发育晚期的功能代谢完善过程。In order to investigate the gene expression pattern of different development stages of rat embryonic pancreas and the reguladon of endocrine cell' s differentiation and metabolism, using Gene Chip Rat Expression Set 230 A and RT-PCR to verify the result. Based on the gene expression profiles, the public databases, such as UCSC, TRANSFAC,NCBI, were explored. In the result BTG2 gene expresses in rat embryonic day 12.5, 15.5, 18.5, adult and new born pancreas were found out. Furthermore, the expression level of E18.5 is nearly five times of any other stages. Its ' function is to promote the cells ' differentiation, anti-proliferation, anti-apoptosis and regulate transcription. In conclusion, BTG2 plays a role in the process of pancreatic endocrine and endocine cells' differentiation and development, it also participates in the process of pancreatic cells' matabolism maturation in late development stages.

关 键 词：胰腺 胚胎 发育阶段 功能代谢 公共数据库 芯片技术 寡核苷酸 发育分化 NCBI 基因信息 调节作用 新生大鼠 分化发育 分泌细胞 高密度 PCR 表达量 晚期 凋亡 

分 类 号：Q954.4[生物学—动物学] S831.5[农业科学—畜牧学]