2004年黑龙江省新城疫病毒分子流行病学分析  

Molecular Epidemiology of Newcastle disease virus in Heilongjiang Province in 2004

作　　者：连宏军[1,2] 刘培欣[1] 闫丽辉[1] 曹殿军[1] 孙建宏[1] 梁宏志 韩正博[1] 冉多良[2] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨150001 [2]新疆农业大学 [3]哈尔滨市兽医卫生防疫站,黑龙江哈尔滨15004

出　　处：《动物医学进展》2005年第7期91-97,共7页Progress In Veterinary Medicine

基　　金：资助:黑龙江省科学技术计划项目(GB02B505)

摘　　要：2004年从黑龙江省部分鸡场疑似新城疫(ND)病死鸡和鸽体内分离到9株NDV。对这9株NDV生物学特性进行测定,并对其F基因包括裂解位点在内的540bp片段进行了克隆、测序和同源性分析。结果表明,9株NDV鸡胚平均死亡时间(MDT)和1日龄雏鸡脑内接种致病指数(ICPI)分别在47.8h～78.4h和1.51～2.00之间;8个分离株在F蛋白裂解位点氨基酸顺序为112RRQKRF117,1个分离株F蛋白裂解位点氨基酸顺序为112RRQRRF117,表明了它们全为ND中、强毒株。同源性分析显示,9株NDV分离株之间核苷酸与推导的氨基酸同源性分别为79.7%～99.7%和79.8%～100%;系统发生树分析表明,6株分离株与台湾株(TW98、TW99和TW2000等)遗传距离较近,可能有共同的起源,为基因Ⅶe型NDV,2株为鸽源NDV基因Ⅵ型,1株与我国特有的F48E9遗传距离最近,为基因Ⅸ型NDV。Nine strains of Newcastle disease virus (NDV), 6 from vaccinated chickens and 2 from pigeons, were isolated from Heilongjiang Province in 2004. All strains were determined to be highly virulent by the mean death time (MDT) of embryonated eggs, and the interacerebral pathogenicity index(ICPI). All NDV isolates were analyzed by partial nucleotide sequencing and phylogenetic analysis of F gene including cleavage site. The results showed that all NDV stains had a homology of 79.7% to 99.7% at nucleotide level,and 79.8% to 100% at amino acid level. Six isolates were grouped into genotype Ⅶe, and they were closer to Taiwan strains than Western Europe. Two strains isolated from pigeons formed a distinctive cluster in genotype Ⅵ.One strain was the closest to F48E9 strain belonged to genotype Ⅸ found in China,suggesting that variants of F48E9 were still present in China.

关 键 词：2004年 黑龙江省 流行病学分析 新城疫 病毒分子 同源性分析 裂解位点 氨基酸同源性 遗传距离 NDV 生物学特性 系统发生树 分离株 死亡时间 F蛋白 病死鸡 F基因 强毒株 核苷酸 分析表 顺序 鸡场 接种 日龄 鸡胚 鸽源 

分 类 号：S858.315.3[农业科学—临床兽医学] U491.31[农业科学—兽医学]