炎性细胞因子在肝缺血-再灌流损伤时心肌组织中的表达  

Expression of TNF-α and IL-1β mRNA in cardiomyocyte following liver ischemia-reperfusion injury in rats and its relationship with cardiomyocyt apoptosis

作　　者：王红梅[1] 张建龙[1] 王新星[1] 孙湛[1] 

机构地区：[1]新疆医科大学基础医学院病理生理教研室,新疆维吾尔自治区乌鲁木齐830054

出　　处：《中华急诊医学杂志》2005年第7期567-570,共4页Chinese Journal of Emergency Medicine

基　　金：新疆维吾尔自治区卫生厅医学科研基金资助项目(2002Y18)

摘　　要：目的探讨大鼠肝缺血-再灌流损伤时TNF-α、IL-1βmRNA在心肌组织中的表达及与心肌细胞凋亡的关系。方法选取健康雄性Wistar大鼠48只,随机分为6组,每组8只。应用免疫组织化学方法和原位杂交方法分别测定心肌组织中TNF-α、IL-1βmRNA的表达;应用原位细胞凋亡法测定细胞凋亡情况。同时观察心肌HE染色。结果I/R组与假手术组TNF-α在心肌组织中的表达,差异有显著性(P<0.01),I/R1h组明显升高,与I/R组比较差异有显著性(P<0.01),I/R2h组、I/R4h组虽然略有波动,但增加差异无显著性(P>0.05)。IL-1βmRNA自I/R组起表达就明显增多,与I组比较差异有显著性(P<0.01);I/R1h组明显升高,与I/R组比较差异有显著性(P<0.05)。细胞凋亡指数I/R组与假手术组比较明显增加(P<0.01),随着再灌流时间的延长细胞凋亡指数逐渐增加。TNF-α、IL-1βmRNA和细胞凋亡指数间均存在正相关(r=0.94,r=0.91;P<0.05)。HE染色后,见心肌部分横纹不清,有炎性细胞浸润,心肌纤维肿胀,心肌水肿。结论肝缺血-再灌流过程中,心肌组织早期就出现TNF-α和IL-1βmRNA的表达,心肌细胞凋亡指数随着再灌流时间的延长逐渐增加,可能与TNF-α和IL-1βmRNA的表达有关;心肌结构出现了病理改变。Objective To study the expression of tumor necrosis factor-α (TNF-α) and interleukin-1βmRNA(IL-1βmRNA) in myocardium tissues after liver ischemia-reperfusion injury in rats, and to explore its relationship with cardiomyocyte apoptosis.Methods Ischemia-reperfusion injury was established by imitation.48 male Wistar rats were randomly divided into 6 groups. The contents of TNF-α and IL-1β mRNA in myocardium tissues were examined by immunohistochemical and in situ hybridization techniques. Myocardium tissues HE staining was observed,and cardiomyocyte apoptosis was studied by TUNEL. Results The expression of TNF-α in myocardium tissues was significantly increased in group I/R1h (P<0.01),but no statistical differences were found between group I/R2h and group I/R4h (P>0.05). The expression of IL-1β mRNA in myocardium tissues started increasing in group I/R1h(P<0.01).Part myocardium become clouding after HE-staining revealed inflammatory cell infiltration,interstitial bleeding,and myocardium edema. There was positive correlation between apoptosis and TNF-α,IL-1βm RNA expression(r=0.94,r=0.91;P<0.05).Conclusion Expressions of TNF-α and IL-1β mRNA is increased in myocardial tissues during liver ischemia-reperfusion injury.Apoptosis index is positively correlated with reperfusion time,which may be related with the expressions of TNF-α and IL-1m RNA.

关 键 词：缺血-再灌流损伤 心肌组织 炎性细胞因子 细胞凋亡指数 TNF-α IL-1β Wistar大鼠 免疫组织化学方法 原位细胞凋亡法 mRNA 心肌细胞凋亡 原位杂交方法 炎性细胞浸润 HE染色 I/R 灌流时间 显著性 心肌纤维 心肌水肿 病理改变 

分 类 号：R575[医药卫生—消化系统]