API2-MALT1融合基因在桥本氏甲状腺炎和甲状腺淋巴瘤中的表达及意义  

Detecting the API2-MALT1 Fusion Gene and Its Significance in Hashimoto's Thyroiditis and Thyroid Lymphomas

作　　者：杨文秀[1,2] 李甘地[1] 周桥[1] 刘卫平[1] 李雷[1] 陈铌[1] 

机构地区：[1]四川大学华西医院病理科,成都610041 [2]贵阳医学院病理学教研室

出　　处：《四川大学学报（医学版）》2005年第4期484-487,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30370600);纽约中华医学基金会(CMB00-722)资助

摘　　要：目的探讨API2-MALT1融合基因在桥本氏甲状腺炎和甲状腺MALT及弥漫大B细胞淋巴瘤(DLBCL)中的表达情况,以了解融合基因表达与甲状腺MALT和弥漫大B细胞淋巴瘤发生及预后的关系。方法用逆转录降落PCR和巢式PCR结合检测8例桥本氏甲状腺炎、12例甲状腺MALT淋巴瘤和3例DLBCL中的API2-MALT1mRNA,5例反应性增生的淋巴结作为检测的阴性对照;通过临床病理和随访资料的分析了解融合基因对两种甲状腺淋巴瘤预后的影响。结果5例反应性增生的淋巴结和8例桥本氏甲状腺炎均未检出融合基因转录,15例甲状腺淋巴瘤检出2例有融合基因转录,1例为MALT淋巴瘤,1例为DLBCL,2例均伴有桥本氏甲状腺炎,临床分期为E期,无淋巴结累及。结论甲状腺MALT淋巴瘤中API2-MALT1融合基因mRNA的检出率虽较低,但不能完全排除API2-MALT1表达在桥本氏甲状腺炎向甲状腺淋巴瘤发展过程中的作用;具有融合基因转录的甲状腺淋巴瘤可能表现出更加惰性的发展过程。Objective To investigate the expression of API2-MALT1 fusion gene mRNA in Hashimoto's thyroiditis, thyroid MALT lymphoma and DLBCL, as well as the correlation between the expression and the pathogenesis and prognosis of two types of thyroid lymphoma. Methods Eight cases of Hashimoto's thyroiditis, 12 cases of MALT lymphoma and 3 cases of DLBCL in thyroid were selected and retrieved. API2-MALT1 transcripts were investigated in all cases by reverse transcript touchdown-PCR and nested PCR. The effect of API2-MALT1 fusion gene on the prognosis of thyroid lymphomas was assessed by analyzing the clinico- pathological features and the follow-up findings. Results The variant 1446/1123 transcript was detected in 2 of thyroid lymphomas (1 MALT lymphoma, 1 DLBCL) but not in 8 cases of Hashimoto's thyroiditis and 5 cases of chronic lymphadenitis. Both of API2-MALT1-positive cases were accompanied with Hashimoto's thyroiditis, showing stageⅠ_E and no involvement of lymph node. Conclusion The incidence of API2-MALT1 transcript in thyroid lymphomas was low; however, the correlation between API2-MALT1 fusion gene and the progression from Hashimoto's thyroiditis to lymphoma could not be excluded; the thyroid lymphoma with API2-MALT1 transcript seemed to develop on a more indolent clinical course.

关 键 词：API2-MALT1融合基因 甲状腺 淋巴瘤 桥本氏甲状腺炎 预后 

分 类 号：R736.1[医药卫生—肿瘤]