部分金属离子及EDTA对瘦肉型猪(PIC344)精液酸性磷酸酶活性与荧光光谱的影响  

Effect of Some Metal Ions and EDTA on Acide Phosphatase from Sperm of PIC Pigs(PIC344)

作　　者：张勇侠[1] 黄新河[1] 刘鑫[1] 国锦琳[1] 李佳[1] 刘克武[1] 

机构地区：[1]四川大学生命科学院

出　　处：《畜牧兽医学报》2005年第7期686-689,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：四川省成都市大熊猫基金项目

摘　　要：研究了K+、Mg2+、Ni2+、Zn2+、Mn2+、Fe2+、Cd2+、Ca2+、Cu2+9种金属离子及EDTA对瘦肉型猪(PIC344)精液酸性磷酸酶(ACPase)活力的影响,发现K+、Mg2+、Ca2+、Ni2+、Mn2+对ACPase有不同程度的激活作用。Cu2+、Cd2+、Fe2+、Zn2+对ACPase有不同程度的抑制作用。同一浓度范围内的金属离子对ACPase的激活作用强弱依次为Mg2+>Ni2+>Mn2+>K+>Ca2+,对ACPase的抑制作用强弱依次为Cu2+>Cd2+>Fe2+>Zn2+。利用双倒数作图法研究了Cu2+、Cd2+对酶的作用,结果表明这2种抑制剂对酶的抑制作用为非竞争性抑制,用Dixon作图法求得其抑制常数Ki值分别为0.58×103mol/L、9.44×103mol/L。研究了Mg2+、Ca2+、Cd2+、Cu2+对酶荧光光谱的影响,结果表明:Mg2+、Ca2+、Cd2+、Cu2+引起酶荧光发射光谱强度降低,最大发射波长不变,说明这些金属离子对酶的构象有不同程度的影响。Acid phosphatase(ACPase) from the sperm of PIC pigs(PIC344) could be activated by these ions of K^+,Mg^(2+),Ca^(2+),Ni^(2+),Mn^(2+), while inhibited by Cu^(2+),Cd^(2+),Fe^(2+),Zn^(2+). The concentrations of these (ions) rang from 1 mmol/L to 5 mmol/L. ACPase was inactivated by EDTA . The ability of metal ions activate the ACPase one by one in order for Mg^(2+)> Ni^(2+)> Mn^(2+)> K^+> Ca^(2+). The ability of metal ions inactivate the ACPase one by one in order for Cu^(2+)> Cd^(2+)> Fe^(2+)> Zn^(2+).Cu^(2+) and Cd^(2+) were selected for determining types of inhibition. The results show that Cu^(2+) and Cd^(2+) were noncompetitive inhibitors by (using) Lineweaver-Burk method.Their Ki values were determined by Dixon method,which respectively were 0.58×10^(-3)mol/L, 9.44×10^(-3)mol/L.The Fluorescent spectras of Mg^(2+),Ca^(2+),Cd^(2+),Cu^(2+) indicate that the fluorescent spectrum strength to decrease, their maximum exit wave-lengths were constant. The results explain that these metal ions influence the conformation of ACPase.

关 键 词：瘦肉型猪 精液 酸性磷酸酶 金属离子 EDTA 

分 类 号：S828.2[农业科学—畜牧学]