日本大耳兔胆道缺血再灌注损伤模型的建立  

Establishment of the model of biliary ischemia-reperfusion in rabbits

作　　者：邵永[1] 马正伟[1] 李昆[1] 梁绍勇[2] 董家鸿[1] 

机构地区：[1]第三军医大学西南医院全军肝胆外科研究所,400038 [2]第三军医大学西南医院普通外科,400038

出　　处：《消化外科》2005年第4期283-287,共5页Journal of Digestive Surgery

基　　金：国家杰出青年科学基金海外青年合作研究基金资助(No.30228030);国家自然科学基金青年科学基金项目资助(No.30400436)

摘　　要：目的建立日本大耳兔胆道缺血再灌注损伤模型,用于肝移植术后胆道并发症及胆道损伤等研究。方法采用无损伤动脉夹联合胆总管及肝总动脉阻断制作胆道缺血模型,以假手术组(SO)及单纯肝动脉阻断组(HAO)作为对照,阻断时间分为2h和3h,各组分别于复流前、复流12h、1、2、3、7d取血测AST、ALT、TBA水平;取胆汁行胆汁TBIL、TBA、GGT测定。术后一周观察动物死亡情况;动物处死后取肝胆组织行石蜡切片HE染色光镜下观察。以单纯肝总动脉阻断组做对照。结果血清、胆汁指标和动物术后7d存活率联合阻断组与单纯肝总动脉阻断组相比差异显著,前者差于后者。随着缺血时间的延长,胆道损伤逐渐加重,病理改变由可逆性损伤转化为不可逆性损伤。结论联合胆总管及肝总动脉阻断胆道缺血再灌注模型胆道缺血完全,重复性好,复制简单,是较好的胆道缺血再灌注损伤模型。Objective To establish a new animal model of biliary ischemia-reperfusion in rabbits. (Methods) Japanese rabbits weighing 210～250 g were devided into 5 groups. Sham operation (SO) and hepatic artery occlusion (HAO) was used in the sham control and HAO group, respectively. Combined clamping of common bile duct and hepatic artery (HBO) were used to produce the (biliary) ischemia-reperfusion animal model. The occlusion lasted for 2 h or 3 h in both HAO and HBO group (HAO 2 h, HAO 3 h, HBO 3 h). Blood samples were taken for measurement of hepatic function (including) alanine transaminase (ALT), asparate transaminase (AST) and total bile acid (TBA). Bile was collected to determine total bilirubin (TBIL), γ-glutamyltransferase (GGT) and TBA. The survival rate and biliary complications were observed 7 days later. Liver and duct were sampled for measurement of histopathological observation. Results There were significant differences in the (hepatic) function, biliary output, animal survival rate and the hepatic pathological changes between group HBO and HAO. Conclusions Combined clamping of CHA and CBD may be an ideal way to produce the model of biliary IR injury in rabbits, which is simple and easy to replicate.

关 键 词：缺血再灌注 胆道 疾病模型 动物 

分 类 号：R-332[医药卫生]