白藜芦醇诱导胃癌原代细胞凋亡(英文)  

Resveratrol induces apoptosis in human primary gastric carcinoma cells

作　　者：周海波[1] 颜云[1] 蔡建庭[1] 杜勤[1] 陈金明[1] 

机构地区：[1]浙江大学附属二院,浙江杭州310009

出　　处：《中国病理生理杂志》2005年第7期1340-1344,共5页Chinese Journal of Pathophysiology

基　　金：Thestudywassupportedbyagrantfromthefoundationforyouthpersonwithabilityofzhejiangprovince(2004QN013)

摘　　要：目的:探讨白藜芦醇诱导胃癌原代细胞发生凋亡的可能性,揭示该凋亡发生与Bcl-2和Bax之间的关系。方法:在体外实验中,采用MTT比色法测定白藜芦醇对胃癌原代细胞的生长抑制率;以透射电镜和TUNEL染色法,定性、定量地研究白藜芦醇与胃癌原代细胞凋亡的关系;通过免疫组织化学法和RT-PCR检测凋亡相关基因bcl-2和bax的表达。结果:白藜芦醇对胃癌原代细胞生长有明显抑制作用,随白藜芦醇浓度增加和培养时间的延长而增强;白藜芦醇诱导胃癌原代细胞出现典型的凋亡细胞形态;TUNEL染色法可见,经白藜芦醇处理24h、48h、72h、96h后,胃癌原代细胞凋亡数明显随时间增加。免疫组化发现经白藜芦醇处理24h、48h、72h、96h后,胃癌原代细胞的Bcl-2蛋白阳性率减少,Bax蛋白阳性率增加。经白藜芦醇处理24、48、72、96h后,RT-PCR检测发现胃癌原代细胞bcl-2和bax的mRNA表达阳性,并显示bcl-2mRNA条带密度随时间的延长而递减,baxmRNA条带密度随时间的延长而增强。结论:白藜芦醇可能通过下调bcl-2的表达和上调bax的表达而诱导胃癌原代细胞发生凋亡。AIM: To investigate the apoptosis in primary gastric cancer cells induced by resveratrol, and the relation between this apoptosis and expression of bcl-2 and bax. METHODS: In in vitro experiments, MTT assay was used to determine the cell growth inhibitory rate. Transmission electron microscopy and TUNEL staining were used to quantitatively and qualitively detect the apoptosis of primary gastric cancer cells before and after the resveratrol treatment. Immunohistochemical staining and RT-PCR was used to detect the expression of apoptosis-regulated gene bcl-2 and bax. RESULTS: Resveratrol inhibited the growth of primary gastric cancer cells in a dose- and time-dependent manner. Resveratrol induced primary gastric cancer cells to undergo apoptosis with typically apoptotic characteristics. TUNEL assay showed that after the treatment of primary gastric cancer cells with resveratrol for 24, 48, 72, 96 hours, the apoptotic indexs were 4.93%±0.19%, 16.74%±0.43%, 27.88%±0.36%, 36.84%±1.07% respectively. Immunohistochemical staining showed that after the treatment of primary gastric cancer cells with resveratrol for 24, 48, 72, 96 hours, the positive rates of Bcl-2 proteins were 20.68%±0.49%, 10.84%±0.33%, 6.80%±0.34%, 3.91%±0.15% and the positive rates of Bax proteins were 19.79%±0.98%, 30.74%±0.85%, 40.14%±1.17%, 60.08%±1.64%. After exposed to resveratrol for 24 h, 48 h, 72 h and 96 h, the density of bcl-2 mRNA decreased progressively with elongation of time and the density of bax mRNA increased progressively with elongation of time by RT-PCR. CONCLUSION: Resveratrol is able to induce the apoptosis in primary gastric cancer. This apoptosis may be mediated by down-regulation of Bcl-2 and up-regulation of Bax.

关 键 词：白藜芦醇 胃肿瘤 细胞凋亡 

分 类 号：R363[医药卫生—病理学]