SeO_2诱导白血病细胞凋亡过程中对细胞内活性氧和Ca^(2+)水平影响  

Selenium dioxide induces leukemia cell apoptosis and changes of intracellular reactive oxygen species (ROS) and Ca^(2+) levels

作　　者：王晓华[1] 魏亚明[2] 章喜明[1] 吴朝霞[1] 郑荣梁[3] 

机构地区：[1]广州医学院生物化学与分子生物学教研室,广东广州510182 [2]南方医科大学南方医院血液科,广东广州510515 [3]兰州大学生命科学院,甘肃兰州730000

出　　处：《中国病理生理杂志》2005年第7期1349-1352,共4页Chinese Journal of Pathophysiology

摘　　要：目的:研究SeO2对急性早幼粒细胞白血病细胞株NB4、红白血病细胞K562、急性粒细胞白血病细胞株HL-60的增生、凋亡、活性氧(ROS)及Ca2+水平等的影响。方法:采用不同浓度SeO2(3-30μmol/L)分别处理3种白血病细胞,用流式细胞术测定细胞凋亡率、细胞中ROS和Ca2+的水平。结果:10和30μmol/LSeO2能抑制3种细胞增生,30μmol/LSeO2作用48h能使54.0%的NB4细胞、46.5%的K562细胞和49.6%的HL-60细胞发生凋亡。同时下调细胞内ROS和Ca2+水平。NB4和HL-60细胞中ROS阳性细胞比例随SeO2浓度增加而减少,K562中只有30μmol/LSeO2才能使ROS出现明显下降。10、30μmol/LSeO2能使NB4和HL-60细胞内Ca2+水平逐步下降。K562中只有30μmol/LSeO2才能使细胞内Ca2+水平出现明显下降。结论:SeO2对3种白血病细胞均有诱导凋亡作用,在凋亡过程中涉及细胞内ROS及Ca2+水平下降。AIM: The effects of selenium dioxide (SeO_2) on proliferation, apoptosis, intracellular reactive oxygen species (ROS) and Ca^(2+) levels in three leukemia cell lines NB4, K562 and HL-60 were investigated. METHODS: Three leukemia cell lines were treated with 3-30 μmol/L SeO_2. Flow cytometry was used to detect apoptosis rate, and analyze the changes of ROS and Ca^(2+) level within cells. RESULTS: SeO_2 at 10 and 30 μmol/L inhibited proliferation in three leukemia cell lines. Treatment with 30 μmol/L SeO_2 for 48 h induced 54.0%, 46.5%, 49.6% apoptosis in NB4, K562, and HL-60 cells, respectively, and also markedly decreased ROS and Ca^(2+) levels among three cell lines. The rate of ROS positive cells in NB4 and HL-60 decreased with the increase in SeO_2 concentrations. ROS was clearly reduced with 30 μmol/L SeO_2 in K562. Ca^(2+) levels were tardily declined with 10, 30 μmol/L SeO_2 in NB4 and HL-60 cells. Ca^(2+) levels were clearly reduced with 30 μmol/L SeO_2 in K562. CONCLUSION: SeO_2 induces apoptosis in three leukemia cells. The declines of intracellular ROS and Ca^(2+) levels are involved in apoptosis induced by SeO_2.

关 键 词：SeO2 细胞凋亡 K562细胞 钙 活性氧 HL-60细胞 

分 类 号：R733.3[医药卫生—肿瘤]