早发性牙周炎患者血清抗牙龈卟啉菌表面抗原抗体滴度和亲和力的研究(英文)  

The titer and avidity of IgG antibody against Porphyromonas gingivalis in sera from patients with early-onset periodontitis

作　　者：张颖[1] 马淑媛[2] 黄世光[1] 张秀琴[1] 吕芳丽 

机构地区：[1]暨南大学医学院口腔系 [2]暨南大学附属第一医院口腔内科 [3]Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-0425, USA

出　　处：《中国病理生理杂志》2005年第7期1353-1355,共3页Chinese Journal of Pathophysiology

基　　金：SupportedbyMedicalScienceFundofGuangdongProvince(No.A2003358)

摘　　要：目的:检测早发性牙周炎患者血清抗牙龈卟啉菌脂多糖IgG抗体的滴度和亲和力,并探讨抗体滴度水平和亲和力之间的相关性。方法:受试者为15名早发性牙周炎患者、16名成人牙周炎患者和14名牙周健康者。检测了血清中抗牙龈卟啉菌脂多糖IgG抗体滴度和亲和力。血清IgG抗体滴度采用酶联免疫吸附试验(ELISA)测定;其亲和力通过二乙醇胺分离,ELISA测定。结果:早发性牙周炎患者和成人牙周炎患者的血清中抗牙龈卟啉菌脂多糖IgG抗体水平显著高于牙周健康者(P<0.01),而在早发性牙周炎患者和成人牙周炎患者之间无显著差异(P>0.05)。早发性牙周炎患者抗体亲和力显著高于成人牙周炎患者和牙周健康组(P<0.01),而成人牙周炎患者和牙周健康组之间无显著差异(P>0.05)。结论:早发性牙周炎患者和成人牙周炎患者的抗体滴度水平和亲和力之间不存在相关性。IgG抗体亲和力可以作为诊断早发性牙周炎的有效参数。AIM: To investigate the titer and avidity of serum immunoglobulin G (IgG) antibody for surface antigens of Porphyromonas gingivalis (P. gingivalis) in sera from patients with early-onset periodontitis (EOP) and evaluate the interrelationship of the antibody titer and avidity. METHODS: 15 patients with early-onset periodontitis, 16 patients with adult periodontitis(AP) and 14 periodontally healthy subjects(HS) participated in this study. Levels and avidities of IgG antibody against P.gingivalis lipopolysaccharide(LPS) were measured. Serum IgG titers against P.gingivalis antigens were measured by enzyme-linked immunosorbent assay (ELISA). IgG avidity was measured by diethylamine dissociation ELISA. RESULTS: Serum IgG antibody levels against P.gingivalis LPS in EOP and AP patients were significantly higher than that of healthy subjects (P<0.01), but there was no significant difference between early-onset periodontitis and adult periodontitis groups (P>0.05). Antibody avidities to P. gingivalis in EOP were significantly higher than that of AP patients and HS group (P<0.01). However,there was no significant difference in antibody avidity between AP patients and HS group (P>0.05). CONCLUSION: These results indicate that there is no correlation between antibody levels and avidities in EOP and AP patients. IgG avidity can be considered as a valuable parameter in the diagnosis of EOP.

关 键 词：牙周炎 紫单胞菌 龈 抗体 脂多糖类 

分 类 号：R781.4[医药卫生—口腔医学]