传入神经阻滞对成年大鼠嗅球钙视网膜蛋白的影响  

Effect of deafferentation on calretinin of adult rat olfactory bulb

作　　者：秦照萍[1] 叶树明[1] 杜继曾[1] 沈公羽[1] 

机构地区：[1]浙江大学生命科学院玉泉校区生物系,浙江杭州310027

出　　处：《中国病理生理杂志》2005年第7期1384-1387,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(编号:30070190)

摘　　要：目的:研究传入神经阻滞对成年SD大鼠嗅球中钙视网膜蛋白(CR)的影响,探讨阻滞的影响是否与动物年龄相关。方法:成年SD大鼠,以ZnSO4灌流单边鼻孔,用免疫组化方法,在损伤后10d、20d、30d、60d,检测嗅球中酪氨酸羟化酶(TH)和CR的表达,以TH表达下降作为传入神经阻滞的标志。结果:损伤后10d、20d、30d和60d,与对照组相比,损伤组嗅球的TH阳性细胞密度和染色强度显著下降,并且从10d、20d到30d逐渐下降,损伤后60d的结果与30d接近。而在所观察的各个阶段,损伤组嗅球的CR阳性细胞数目和染色强度与对照组相比,均无显著差异。结论:大鼠嗅球中CR的表达不受传入神经阻滞的影响,且阻滞对CR表达的影响与动物年龄无关。AIM: To investigate the effect of deafferentation on the expression level of calretinin (CR) in adult Sprague Dawley rat olfactory bulb (OB). METHODS: Adult Sprague Dawley rats were given a single intranasal irrigation with ZnSO_4. A specific period (10, 20, 30 and 60 days, respectively) after the treatment, immunoreactivities of tyrosine hydroxylase (TH) and calretinin of OB were examined by using immunohistochemistry. Down-regulation of TH expression level was regarded as a mark of deafferentation. RESULTS: 10, 20, 30 and 60 days after ZnSO_4 treatment, staining intensity and density of TH-immunoreactive cells of OB significantly reduced and the degree of reduction was correlated with the time of treatment before 30 days, but the result of 60 d was similar to that of 30 d. There was no obvious change in the density of CR-immunopositive cells after the same treatment. CONCLUSION: Expression of CR in adult rat OB was not affected by deafferentation.

关 键 词：大鼠 嗅球 神经肌肉阻滞 钙视网膜蛋白 

分 类 号：R363[医药卫生—病理学]