体外血小板活化因子对神经元和星形胶质细胞的作用  

Effects of platelet-activating factor on cultured neurons and astrocytes

作　　者：张敬各[1] 李树清[1] 李凡[1] 

机构地区：[1]昆明医学院基础医学院病理生理教研室,云南昆明650031

出　　处：《中国病理生理杂志》2005年第7期1393-1396,共4页Chinese Journal of Pathophysiology

基　　金：云南省自然科学基金资助项目(No.2004C0043M)

摘　　要：目的:研究血小板活化因子(PAF)对神经元活力及星形胶质细胞胶质纤维酸性蛋白(GFAP)表达的影响。方法:分别取BALB/c胎鼠和新生小鼠大脑皮层,纯化培养神经元和星形胶质细胞,设正常对照组和实验组,实验组中分别加入4、8和16μmol/L的PAF并分别作用4h、24h和72h,用MTT法和免疫组织化学方法测定神经元活力和星形胶质细胞表达GFAP的平均灰度值。结果:PAF作用后,神经元活力降低;星形胶质细胞数量减少,但存活细胞GFAP表达增加。两者均呈浓度依赖关系。结论:PAF不仅直接作用于神经元,且可通过作用于星形胶质细胞间接影响神经元的存活。AIM: To observe the effect of platelet-activating factor (PAF) on cultured neuronal viability and glial fibrillary acidic protein (GFAP) expression in cultured astrocytes. METHODS: Neurons and astrocytes obtained from the brain cortex of the embryo and newborn mice respectively were cultured and purified, and they were divided into the control and experimental groups. PAF was added into the experimental groups at concentrations of 4, 8 and 16 μmol/L. Each group was cultured for 4 h, 24 h and 72 h, respectively. MTT method and immunohistochemistry were used to observe the neuronal viability and GFAP expression in astrocytes, respectively. RESULTS: During different time after adding PAF at different concentrations into cultured neurons and astrocytes, respectively, neuronal viability declined, and the number of astrocytes decreased, but GFAP expression in survival astrocytes increased. The effects were shown to be in a concentration-dependent manner. CONCLUSION: PAF decreases the neuronal viability directly and influences the neuronal survival indirectly by astrocytes.

关 键 词：血小板活化因子 细胞培养 神经元 星形细胞 神经胶质原纤维酸性蛋白质 

分 类 号：R363[医药卫生—病理学]