线粒体DNA缺失对人肺癌细胞生长和侵袭能力的影响  

Mitochondrial DNA deletion on the growth and invasiveness of human lung cancer cells

作　　者：凌贤龙[1] 陆应麟[2] 杜芝燕[2] 

机构地区：[1]第三军医大学新桥医院消化内科,重庆400037 [2]军事医学科学院基础医学研究所病理生物学研究室,北京100850

出　　处：《第三军医大学学报》2005年第14期1453-1455,共3页Journal of Third Military Medical University

基　　金：国家重点基础研究发展规划资助项目("973"项目;2002CB513105);国家自然科学基金资助项目(30200141)~~

摘　　要：目的探讨线粒体DNA损伤对人肺癌细胞生长和侵袭能力的影响。方法采用溴化乙锭处理获得线粒体DNA缺失细胞;软琼脂集落形成实验检测细胞集落形成能力;Transwell侵袭实验检测细胞侵袭能力;MTT方法测定细胞长状态。结果线粒体DNA缺失细胞较之于其母本细胞系,具有更强的集落形成和侵袭能力,以及更明显的生长优势结论线粒体损伤可能参与了肺癌细胞恶性表型的形成。Objective To explore the relationship between mitochondrial DNA deletion and malignant phenotypes of human lung cancer cells. Methods Two rhoˉ derivatives of 95C and 95D were generated by treating the cultured cells with ethidium bromide. Agarose colony formation assays and Transwell invasion assays were carried out to detect the phenotypes of colony formation and invasiveness of the cultured cells, respectively. Cell growth was determined by MTT. Results The partially mtDNA-deleted cells exhibited stronger capacity of colony formation and invasiveness, and faster growth rates than their respective parental cell lines. Conclusion Mitochondrial DNA deletion might play a role in the formation of malignant phenotypes of human lung cancer.

关 键 词：线粒体 线粒体DNA 肺癌 恶性表型 

分 类 号：Q527[生物学—生物化学] R730.23[医药卫生—肿瘤]