兔胚胎全层视网膜移植到大鼠视网膜下腔的研究  

Transplantation of rabbit embryonic full-thickness retina into the subretinal space of rats

作　　者：余涛[1] 阴正勤[1] 王仕军[1] 

机构地区：[1]第三军医大学西南医院全军眼科中心,重庆400038

出　　处：《第三军医大学学报》2005年第13期1335-1337,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目(30270457)~~

摘　　要：目的观察兔胚胎全层视网膜移植物,包括神经上皮和视网膜色素上皮(retinalpigmentepithelium,RPE),在正常Wistar大白鼠和视网膜变性(Royalcollegeofsurgeons,RCS)大鼠视网膜下腔的发育状况,从而探讨异种胚胎全层视网膜移植方法的可行性。方法采用17只孕3周兔胚胎作为移植供体,酶法分离得到全层视网膜片(包括神经上皮和RPE),将其移植到20只Wistar大鼠和14只RCS大鼠的右眼视网膜下腔;分别于术后3d、1、2、4周处死宿主,应用视网膜组织切片进行HE染色和免疫组化染色,观察供体视网膜的生长发育状况。结果兔胚胎视网膜能在两种大鼠视网膜下腔发育为典型的“玫瑰花结”及视网膜各层结构。结论兔胚胎视网膜在大鼠视网膜下腔能进行分化和发育。异种胚胎全层视网膜移植是可行的,但移植方法还需进一步探索和完善。Objective To study the transplantation of embryonic full-thickness retina, including neural retina and retinal pigment epithelial (RPE), into the subretinal space of rats and to investigate the development of the grafts. Methods A total of 34 eyes from 3-week-old pigmented rabbit embryos were treated with the dispase to obtain pieces of the full-thickness retina, and then grafts were injected into the subretinal space of the right eyes of 20 Wistar rats and 14 RCS (Royal college of surgeons) rats. Host rats were sacrificed at 3, 7, 14, and 28 d after transplantation. Sections were stained with hematoxylin eosin and immunohistochemistry. Results The rabbit embryonic retina was capable of developing and differentiating in the subretinal space of the host rats. Conclusion The rabbit embryonic retina is capable of developing and differentiating in the subretinal space of host rats. Transplantation of xenografts of embryonic full-thickness retina is practicable, but the methods of transplantation need to be improved.

关 键 词：胚胎视网膜 移植 玫瑰花结 

分 类 号：R-332[医药卫生] R617