丙烯酰胺诱导小鼠淋巴瘤细胞tk基因突变的研究  

Acrylamide Inducetk Gene Mutation in Mouse Lymphoma Cells

作　　者：袁健[1] 刘胜学[1] 曹佳[1] 熊燕[1] 

机构地区：[1]第三军医大学军事预防医学院卫生毒理学教研室,重庆400038

出　　处：《癌变．畸变．突变》2005年第4期202-205,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金资助项目(No.30100153;No.30471476)

摘　　要：背景与目的:研究丙烯酰胺(Acrylamide,AA)对小鼠淋巴瘤L5178Y3.2.7c-tk+/-细胞tk基因突变频率的影响。材料与方法:使用丙烯酰胺对L5178Y细胞进行梯度染毒,分别进行细胞毒性,细胞接种效率,相对悬浮生长率和突变频率的测定。结果:随着AA剂量的增加,L5178Y细胞的相对存活率和悬浮生长率均明显降低。在150～750μg/ml范围内诱导L5178Y细胞tk基因的突变频率高于自发突变频率1～9倍,表明具有较强的致突变性。结论:AA对L5178Y细胞具有明显的细胞毒性,并可以诱导tk基因的突变。BACKGROUND&AIM: To investigate the effect of acrylamide(AA)on the tk gene of mouse lymphoma cells. MATERTAL AND METHODS: L5178Y cells were treated with AA at different concentrations.Determination of cytotoxicity,cell inoculation efficiency,relative survival rate and mutation frequency was performed. RESULTS: The relative survival rate and suspension growth rate of L5178Y cells decreased significantly with the increasing doses of AA.The mutation frequency of tk gene induced by AA(150～750μg/ml)was1～9times higher than that of spontaneous mutation frequency of L5178Y cells. CONCLUSION: AA have obvious cytotoxic and mutagenic effects on tk gene in L5178Y cell.

关 键 词：丙烯酰胺 tk位点 致突变作用:L5178Y细胞 

分 类 号：Q754[生物学—分子生物学] R733.4[医药卫生—肿瘤]