VEGF-C及其受体Flt-4在原发性非小细胞肺癌中的表达及意义  

Expression of VEGF-C and Its Receptor Flt-4 in Primary Non-small Lung Cancer and Clinical Significance

作　　者：郑晓华[1] 赵炜[1] 张培义[1] 靳建亚[1] 

机构地区：[1]解放军第二零二医院综合科,辽宁沈阳110003

出　　处：《癌变．畸变．突变》2005年第4期232-235,共4页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：背景与目的:探讨血管内皮生长因子C(VascularendothelialgrowthfactorC,VEGF-C)及其受体Flt-4(Fms-liketyrosinekinase4)在人非小细胞肺癌(Non-smallcelllungcancer,NSCLC)组织中的表达及与临床意义。材料与方法:采用半定量的RT-PCR及免疫组化法检测40例NSCLC、12例癌旁组织及免疫组化法(S-P法)检测60例NSCLC原发灶组织、10例癌旁组织、32例伴转移的淋巴结组织中VEGF-C、Flt-4的表达。结果:RT-PCR法:NSCLC中VEGF-CmRNA及Flt-4mRNA的相对含量均明显高于癌旁组织;与淋巴结转移正相关;与肺癌的组织类型、病理分级无关。VEGF-CmRNA与肺癌的TNM分期正相关。S-P法:VEGF-C和Flt-4在转移的淋巴结癌细胞中、NSCLC和癌旁组织中的表达各组间差异有显著意义;VEGF-C表达与肺癌TNM分期正相关;VEGF-C和Flt-4表达与淋巴结转移正相关,与肿瘤组织类型、病理分级无关;NSCLC中Flt-4阳性微脉管数在淋巴结转移组明显高于无淋巴结转移组,差异有显著意义。结论:在NSCLC组织中VEGF-C基因水平上调,是由肺癌细胞分泌,并通过自分泌方式作用于细胞膜上的Flt-4受体,与非小细胞肺癌的发生有一定关系;VEGF-C与肿瘤恶性进展有关;VEGF-C与NSCLC中淋巴管的生成及淋巴结转移密切相关;VEGF-C表达可做为肺癌患者判断淋巴转移的估计指标之一。BACKGROUND&AIM: To study the expression of vascular endothelial growth factor C(VEGF-C)and Fms-like tyrosine kinase4(Flt-4)in non-small cell lung cancer(NSCLC)and the clinical significance. MATERIALS AND METHODS: Detection of VEGF-C and its receptor Flt-4by RT-PCR in12paracancer tissues and40NSCLC cases and by Strept avidin-biotin peroxidase(S-P)immunohistochemical staining in10paracancer tissues,60NSCLC cases and32metastaticlymph nodes of NSCLC. RESULTS: RT-PCR detection result:the relative contents of VEGF-C mRNA and Flt-4mRNA in NSCLC were higher than that in paracancer tissues and had positive correlation with lymph node metastasis.The expression of VEGF-CmRNA had positive correlation with tumor TNM stage.S-P detection result:The difference of positive expression rate of VEGF-C and Flt-4in NSCLC and paracancer tissues and lymphatic node metastasis was significant respectively.The expression of VEGF-C of NSCLC correlated with the TNM stages.The expression of VEGF-C and Flt4of NSCLC was closely related to lymph node metastasis.VEGF-C,Flt-4had no relation with the histological type and pathologic grade.The microvessels of positive Flt-4in lymphatic node with metastasis were higher than that in lymphatic node without metastasis.The difference was significant. CONCLUSIONS: VEGF-C,secreted by lung cancer cells,up-regulates the expression of Flt-4in NSCLC and takes its effect via Flt-4receptors in cell membrane by autocrine.It contributes to the genesis of NSCLC.Expression of VEGF-C can serve as the indication of lymphatic metastasis in lung cancer.

关 键 词：非小细胞肺癌 淋巴转移 血管内皮生长因子 

分 类 号：R734.2[医药卫生—肿瘤]