双加氧酶活力对细菌降解菲的指示作用  

Indicative function of dioxygenase activities on the degradation of phenanthrene by bacteria

作　　者：许华夏[1] 李培军[1] 巩宗强[1] 刘宛[1] 钟鸣[2] 陈素华[1] 

机构地区：[1]中国科学院沈阳应用生态研究所,沈阳110016 [2]沈阳农业大学,沈阳110161

出　　处：《生态学杂志》2005年第7期845-847,共3页Chinese Journal of Ecology

基　　金：国家自然科学基金重点项目(20337010);国家"863"计划项目(2004AA649060);国家自然科学基金资助项目(20277040)。

摘　　要：在液体培养基中选用两种石油降解细菌进行菲降解实验,研究了菲降解率和双加氧酶活力的变化。结果表明,菲降解率受其浓度的影响,当菲浓度为100mg·L-1时,其降解率为最高。而菲浓度高于100mg·L-1时,其降解率下降。实验发现在菲浓度为50～250mg·L-1条件下,细菌的双加氧酶活力与其菲的降解率存在较好的相关性,对细菌降解菲具有指示作用,可将双加氧酶活力作为菲降解率变化的评价指标。Experiments on degradation of phenanthrene by two species of petroleum-degrading bacteria and the change of dioxygenase activities were carried out.The results indicated that when the phenanthrene concentration was 100 mg·L^(-1), phenanthrenee degradation was the highest.When the phenanthrene concentration was higher than 100 mg·L^(-1),the degradation decreased.There was a significant correlation between phenanthrene degradation and dioxygenase activities when the phenanthrene concentration ranged from 50 to 250 mg·L^(-1).The dioxygenase activities could be used as an effective assessment index of phenanthrene degradation process and intensity.

关 键 词：双加氧酶 细菌 降解 菲 

分 类 号：X172[环境科学与工程—环境科学]