RNAi抑制XJ-160病毒复制的研究  

Inhibition of XJ-160 virus replication by RNAi

作　　者：邓娟[1] 杨益良[1] 付士红[1] 王力华[1] 金冬雁[1] 梁国栋[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所病毒基因工程国家重点实验室,北京100052

出　　处：《中华微生物学和免疫学杂志》2005年第6期496-500,共5页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金(No:39970037)

摘　　要：目的通过建立RNA干扰(RNAinterference,RNAi)抑制XJ160病毒复制的模型,在序列特异性、位置效应和量效关系等方面,探讨RNAi对XJ160病毒复制的影响,为进一步研究RNAi的抗病毒作用和机制奠定基础。方法按照siRNA(shortinterferencingRNA)的设计要求合成XJ160病毒特异siRNA,脂质体法转染BHK21细胞后感染XJ160病毒,通过测定病毒滴度、蛋白表达量及XJ160病毒RNA合成研究siRNA对XJ160病毒复制的抑制。结果成功建立了RNAi抑制XJ160病毒复制的模型,探讨了RNAi抑制该病毒复制作用的特点。结论外源导入的siRNA能够抑制XJ160病毒的复制,并且这种抑制作用具有明显的序列特异性、位置效应和存在量效关系等特点;siRNA是通过降解病毒RNA实现抑制病毒复制作用的。Objective By construct the model of RNAi-mediated inhibition of XJ-160 virus replication to study RNAi-mediated inhibition of XJ-160 virus replication on aspect of sequence, positional and dose dependent, to further research on effect and mechanism of RNAi on antiviral effects. Methods Synthesized the XJ-160 virus special short interferencing RNA (siRNA) according to the requiring of siRNA design. siRNA was transfected into BHK-21 cells via liposomes, then infected the BHK-21 cells by XJ-160 virus. Through detecting the titer, protein expression and RNA synthesizing of XJ-160 virus, research on siRNA -mediated inhibition of XJ-160 virus replication. Results Constructed the model of RNAi-mediated inhibition of XJ-160 virus replication successfully, and discussed the character of it. Conclusion siRNA can inhibit the replication of XJ-160 virus. It has sequence, positional and dose dependent. And it is carried out by digested the virus RNA.

关 键 词：病毒复制 RNAi XJ-160病毒 siRNA BHK-21细胞 序列特异性 量效关系 位置效应 RNA干扰 抗病毒作用 病毒RNA 设计要求 脂质体法 病毒滴度 合成研究 抑制病毒 步研究 表达量 抑制作 模型 

分 类 号：R346[医药卫生—基础医学]