中药益髓生血颗粒对β-珠蛋白生成障碍性贫血患儿基因表达的影响  

Effect of Yisui Shengxue Granule on Gene Expression in Children with β-Thalassemia

作　　者：柴立民[1] 吴志奎[1] 张新华[2] 蔡辉国[3] 

机构地区：[1]中国中医研究院广安门医院分子生物学研究室,北京100053 [2]中国人民解放军第303医院血液病研究室 [3]中国医学科学院实验血液学国家重点实验室

出　　处：《中国中西医结合杂志》2005年第7期591-594,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.30171199);国家自然科学基金重大研究计划(No.90409003)

摘　　要：目的探讨益髓生血颗粒治疗β珠蛋白生成障碍性贫血的作用机制。方法观察20例患儿治疗前后血红蛋白(Hb)、红细胞(RBC)、网织红细胞计数(Ret)和胎儿血红蛋白(HbF)的改变;选取3例有效病例,通过mRNA差异显示技术(DDRTPCR)研究益髓生血颗粒对β珠蛋白生成障碍性贫血患儿骨髓有核细胞的基因表达差异。结果与治疗前比较治疗后患儿各项血液学参数均有提高(P<0.05或P<0.01);患儿骨髓有核细胞的铁蛋白基因表达明显降低。结论益髓生血颗粒可以改善患儿的临床症状,明显降低患儿铁蛋白基因的表达,有效缓解患儿体内铁的蓄积,这可能是益髓生血颗粒治疗本病的分子机制之一。ObjectiveTo investigate the mechanism of Yisui Shengxue granule (YSG) in children with β-thalassemia. MethodsThe changes of hemoglobin (Hb), red blood cells (RBC), reticulocyte (Ret) and fetal hemoglobin (HbF) of 20 children with β-thalassemia were measured before and after treatment. The effect of YSG on gene expression in myelo-karyocytes in 3 selected children treated effectively by YSG was determined by using DDRT-PCR. ResultsThe hematologic parameters were significantly enhanced and the ferritin gene expression declined after treatment respectively, as compared with those before treatment, the difference were significant (P<0,01 or P<0.05). ConclusionYSG could improve the clinical symptoms of children with β-thalassemia. One of its therapeutic mechanisms may be its action in decreasing ferritin gene expression so as to effectively relieve the accumulation of iron in body.

关 键 词：中药 益髓生血颗粒 Β-珠蛋白生成障碍性贫血 患儿 基因表达 中医药疗法 

分 类 号：R272[医药卫生—中医儿科学]