参芪扶正注射液诱导成人间质干细胞治疗脑梗塞的实验研究  

Experimental Research on Effect of Human Mesenchymal Stem Cells Induced by Shenqi Fuzheng Injection in Cerebral Infarction

作　　者：姚晓黎[1] 张成[1] 卢锡林[1] 冯善伟[1] 邓宇斌[2] 刘祖国[3] 

机构地区：[1]中山大学附属第一医院神经科,广州510080 [2]中山大学病理生理教研室 [3]中山大学眼科中心

出　　处：《中国中西医结合杂志》2005年第7期629-632,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.30270732;30170337);卫生部临床学科重点项目(No.2001321);广东省科技计划项目(No.ZKM055015);广东省科技计划项目重大专项(No.2003A3020106)

摘　　要：目的观察参芪扶正注射液(简称参芪液)诱导成人间质干细胞(hMSCs)在脑梗塞大鼠脑内的分化及其对模型大鼠神经功能的影响。方法建立左大脑中动脉梗塞模型大鼠,将在体外扩增的hMSCs经参芪液诱导0.5h后注入模型大鼠脑内,观察hMSCs在大鼠脑内的存活、分化及对模型大鼠神经功能的改变。结果hMSCs移植排斥反应低,可在大鼠脑内存活6周以上;移植前后脑梗塞面积无明显变化;免疫组化表明hMSCs在大鼠脑内表达人神经元特异性烯醇化酶(NSE)、神经丝蛋白(NF)和胶质纤维酸性蛋白(GFAP);模型大鼠的肢体运动功能和触觉功能均有改善。结论参芪液可诱导hMSCs在体内分化为神经元,hMSCs有可能成为治疗脑梗塞的理想的种子细胞。ObjectiveTo investigate the effect of Shenqi Fuzheng injection (SFI) in inducing differentiation of human mesenchymal stem cells (hMSCs) in brain stem and its effect on nervous function in model rats of cerebral infarction. MethodsMiddle cerebral artery occlusion model rats were made, and hMSCs was injected into their brain after being amplified in vitro and incubated with SFI for 0.5h, then the survival, migration and differentiation of hMSCs in brain stem as well as the change of nervous function in model rats were observed. ResultsThe post-transplantation reject reaction to hMSCs was low, it could survive as long as 6 weeks or more. No difference in area of infarction was shown before and after transplantation. Immunohistochemical staining showed that hMSCs expressed human neuron specific enolase (NSE), neurofilament (NF) and glial fibrillary acid protein (GFAP). The limb-kinetic function and tactile perception were improved in the model rats. ConclusionSFI can induce hMSCs differentiate into neurons in vivo, and hMSCs may be the ideal germinal cells for treating cerebral infarction.

关 键 词：参芪扶正注射液 诱导 间质干细胞 治疗 脑梗塞 实验研究 

分 类 号：R277.7[医药卫生—中医学]