组胺H_2受体激动剂4－甲基组胺及其拮抗剂雷尼替丁对HL－60白血病细胞增殖的作用  

THE EFFECTS OF HISTAMINEH_2 RECEPTOR AGONIST4-METHYLHISTAMINE AND ANTAGONIST RANITIDINE ON THE PROLIFERATION OF HL-60 LEUKEMIA CELLS

作　　者：何群[1] 徐有恒[1] 

机构地区：[1]湖南医科大学血液生理学研究室

出　　处：《中国应用生理学杂志》1995年第4期294-298,共5页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金资助课题

摘　　要：在ＨＬ－６０白血病细胞的体外培养中，组胺Ｈ＿２受体激动剂４－甲基组胺（１０￣（－８）－１０￣（－４）ｍｏｌ／Ｌ）对ＨＬ－６０细胞的增殖具有轻微的抑制作用，而组胺Ｈ＿２受体拮抗剂雷尼替丁（１０￣（－８）－１０￣（－４）ｍｏｌ／Ｌ）对ＨＬ－６０细胞的增殖具有较强的抑制作用。但用１０￣（－６）ｍｏｌ／Ｌ的雷尼替丁预处理ＨＬ－６０细胞则可部分地拮抗４－甲基组胺（１０￣（－８）－１０￣（－４）ｍｏｌ／Ｌ）对ＨＬ－６０白血病细胞的抑制作用。The results obtained from liquid suspension culture,semisolid culture and ￣3H-TdR incorpo-ration into HL-60 leukemia cells indicated that histamine H_2 receptor agonist 4-methylhistamine(4-MH) had slight suppressive effect on HL-60 leukemia cells when the 4-MHdoses were 10￣(-8)-10￣(-4)mol / L.The IC_50 of 4-MH in HL-60 colony culture was 1.74 ×10￣(-5)mol / L. Histamine H_2 receptor antagonist ranitidine exerted its suppressive effect onHL-60 leukemia cells when ranitidine 10￣(-8)mol / L to 10￣(-4)mol / L were added.The IC_50 ofranitidine in HL-60 colony culture was 4.24×10￣(-8)mol / L.Therefore, histamine H_2 receptor an-tagonist ranitidine exerted greater suppressive effect on HL-60 leukemia cells than histamine H_2receptor agonist 4-MH did.When HL-60 leukemia cells were pretreated with ranitidine10￣(-6)mol / L for 0.5h,which had no supressive effect on HL-60 cells,the inhibitory effect of4-MH(10￣(-8)-10￣(-4)mol / L) on HL-60 leukemia cells could be antagonized to some extent.Thesephenomena suggest that histamine H_2 receptor agonist 4-MH and its antagonist ranitidine mayexert their suppressive effects on HL-60 leukemia cells through different mechanisms whichshould be clarified in further experiments.

关 键 词：白血病 组胺 激动剂 拮抗剂 雷尼替丁 药理学 

分 类 号：R733.705[医药卫生—肿瘤] R965[医药卫生—临床医学]