胃癌中COX-2及NF-κB的表达与其病理特征的关系  

Study on correlation between expression of COX-2, NF-κB and pathology of gastric cancer

作　　者：蔡跃芳 张明亮[2] 严悦卿[2] 陈韬 吴浪庭 骆训武 

机构地区：[1]广东东莞黄江医院内科,广东东莞523750 [2]湖南南华大学附二医院,湖南衡阳421001

出　　处：《中国现代医学杂志》2005年第12期1830-1833,共4页China Journal of Modern Medicine

摘　　要：目的研究胃癌(gastriccancer,GC)中环氧合酶-2(cyclooxygenase-2,COX-2)、核因子-κB(nuclearfactorκappaB,NF-κB)的表达规律与病理特征的关系。方法采用免疫组织化学染色法(SABC法)和末端脱氧核苷酸转移酶(TDT)介导的dUTP缺口标记法(TUNEL)检测15例正常胃黏膜(normalgastricmucosa,NGM)和40例GC组织中COX-2、NF-κBp65表达情况。结果GC中COX-2、NF-κBp65的阳性表达分别为67.5%、62.5%,NGM组则为13.3%、6.70%,GC组和NGM组差别有显著性(P<0.01)。COX-2、NF-κBp65在低分化GC中表达显著高于高分化型,且后者还与GC病期有关(P<0.05)。COX-2、NF-κBp65与GC淋巴结转移、血管浸润均密切相关(P<0.05)。结论COX-2、NF-κB的表达水平对评价GC的恶性程度、有无转移和预后有一定参考价值。[Objective] To study expression of cyclooxygenase-2 (COX-2), nuclear factor-kappa B(NF-κB) in the gastric cancer and their relation with pathological grades and clinical stages. [Methods] Gastric tissue specimens were obtained from 15 cases with normal gastric mucosa (NGM), and 40 with gastric cancer (GC). Expression of COX-2 and NF-κB p65 were detected by immunohistochemistry ( with SABC method ). [Results] Positive rates of COX-2 and NF-κB p65 in GC were 67.5% and 62.5%, 13.3% and 6.7% in NGM respectively. There was a significant difference between GC and NGM(P <0.05). Expression of COX-2 and NF-κB p65 in poorly differentiated cancer was markedly higher than that in well differentiated cancer (P <0.05); furthermore, NF-κB p65 was related to the clinical stages of GC. Expression of COX-2 and NF-κB p65 were all closely correlated with lymphnode,,metastasis and vascular invasion (P <0.05). [Conclusion] Expression levels of COX-2 and NF-κB may be helpful for evaluating malignant degree of GC, lymphnode, metastasis and prognosis.

关 键 词：COX-2 病理特征 胃癌 末端脱氧核苷酸转移酶 免疫组织化学染色法 NF-kB cancer factor Kappa SABC法 淋巴结转移 p65 环氧合酶 表达规律 dUTP 表达情况 阳性表达 高分化型 血管浸润 恶性程度 参考价值 GC 核因子 胃黏膜 

分 类 号：R735.2[医药卫生—肿瘤] R734.2[医药卫生—临床医学]