谷氨酰胺对人肝癌细胞系SMMC-7721增殖影响的体外研究  

Effects of Glutamine on Proliferation of Human Hepatoma Cell Line SMMC-7721 in vitro

作　　者：徐仁应[1] 万燕萍[1] 沈婉蓉[1] 阮奕[1] 陈之琦[1] 唐激文[1] 

机构地区：[1]上海第二医科大学附属仁济医院临床营养科,200001

出　　处：《胃肠病学》2005年第3期148-152,共5页Chinese Journal of Gastroenterology

摘　　要：背景谷氨酰胺与肿瘤的发生密切相关,但其促体外培养肿瘤细胞增殖的机制目前尚不明确。目的探讨谷氨酰胺在体外影响人肝癌细胞系SMMC鄄7721增殖的可能机制。方法人肝癌细胞系SMMC鄄7721与不同浓度(0、0.5、1.0、2.0、4.0、8.0、16.0mmol/L)的谷氨酰胺共培养24h。四甲基偶氮唑蓝(MTT)比色法检测细胞增殖情况,流式细胞仪检测细胞周期变化,逆转录聚合酶链反应(RT鄄PCR)检测热休克蛋白(HSP)70的表达。结果谷氨酰胺能显著促进人肝癌细胞系SMMC鄄7721的增殖,谷氨酰胺浓度为2.0mmol/L时其促肿瘤生长的作用最强,而后又逐步下降。添加谷氨酰胺的肿瘤细胞,S期细胞比(SPF)和细胞增殖指数(PI)以及HSP70的表达较无谷氨酰胺者显著增加,且其作用随谷氨酰胺浓度的增加而增强,至浓度为2.0mmol/L时其作用最强,而后逐渐下降。结论谷氨酰胺可诱导体外培养的人肝癌细胞系SMMC鄄7721HSP70表达增高,可能是其促肿瘤生长的作用机制之一。Background: Glutamine is closely related to tumorigenesis, but its mechanism in promoting the proliferation of cultured tumor cells in vitro remains unclear. Aims: To investigate the possible mechanism of glutamine on the proliferation of human hepatoma cell line SMMC-7721 in vitro. Methods: Human hepatoma cell line SMMC-7721 was cultured with different concentrations of glutamine (0, 0.5, 1.0, 2.0, 4.0, 8.0, 16.0 mmol/L) for 24 hours. The cell proliferation was detected by methyl thiazolyl tetrazolium (MTT) colorimetric assay; the cell cycle was measured by flow cytometry; the expression of heat shock protein (HSP) 70 was detected by reverse transcriptase polymerase chain reaction (RT-PCR). Results: Glutamine promoted the proliferation of human hepatoma cell line SMMC-7721 significantly, which was reflected by increased S-phase fraction and the proliferation index (PI). The expression of HSP70 in glutamine-treated cells was significantly higher as compared with that in non-treated cells. The expression of HSP70 was consistent with glutamine concentration. Both the cell proliferation and HSP70 expression showed a dose-dependent relationship with the concentration of glutamine. Glutamine at a concentration of 2.0 mmol/L showed a maximum effect. Conclusions: Glutamine can up-regulate the expression of HSP70 in human hepatoma cell line SMMC-7721 in vitro, which may be one of its the mechanisms of growth promotion of cancers.

关 键 词：谷氨酰胺 肝癌细胞 SMMC-7721 细胞增殖 肿瘤 消化系统 

分 类 号：R735.7[医药卫生—肿瘤]