雷公藤内酯醇对大鼠实验性结肠炎的治疗作用  

Therapeutic Effects of Triptolide on Trinitro-benzene-sulfonic Acid-induced Experimental Colitis in Rats

作　　者：兰雷[1] 陈垦[2] 龙友明[1] 王晖[3] 

机构地区：[1]广东医学院附属医院消化内科,524000 [2]广东药学院附属第一医院 [3]广东医学院动物中心

出　　处：《胃肠病学》2005年第3期157-160,共4页Chinese Journal of Gastroenterology

基　　金：广东医学科学基金立项课题(No:A2003554)资助

摘　　要：背景炎症性肠病(IBD)病程迁延,传统的药物治疗效果均不理想,寻找新型而有效的药物一直是该领域研究的热点。目的观察不同剂量雷公藤内酯醇对大鼠实验性结肠炎的治疗作用和不良反应。方法60只Sprague鄄Dawley大鼠随机分为6组:模型组、低剂量(0.1mg/kg)雷公藤内酯醇组、中剂量(0.2mg/kg)雷公藤内酯醇组、高剂量(0.4mg/kg)雷公藤内酯醇组、丙二醇(20%,5ml/kg)对照组和地塞米松(0.2mg/kg)对照组。应用三硝基苯磺酸(TNBS)/乙醇溶液诱导产生大鼠结肠炎模型,以相应药物处理2周后处死大鼠。处死大鼠前1天行白细胞计数检查,处死后直接称取胸腺重量。计算结肠溃疡面积和湿干比;测定结肠黏膜超氧化物歧化酶(SOD)和髓过氧化物酶(MPO)活性;采用酶联免疫吸附(ELISA)法测定结肠组织白细胞介素(IL)鄄1和肿瘤坏死因子(TNF)鄄α水平。结果不同剂量雷公藤内酯醇治疗后,能显著减少大鼠结肠黏膜的溃疡面积,降低结肠湿干比;显著升高结肠黏膜SOD活性、降低MPO活性;显著降低IL鄄1和TNF鄄α的水平;使大鼠胸腺明显缩小,白细胞计数显著减少。结论雷公藤内酯醇对TNBS/乙醇溶液诱导的大鼠实验性结肠炎具有显著的治疗作用,同时剂量大时亦有明显的不良反应。Background: Inflammatory bowel disease has a prolonged course and its conventional treatment is still unsatisfact-ory. Looking for a new and effective drug is always the hotspot in this field. Aims: To investigate the therapeutic and adverse effects of different dosages of triptolide on trinitro-benzene-sulfonic acid (TNBS)-induced experimental colitis in rats. Methods: Sixty male Sprague-Dawley rats were randomly divided into six groups: model group, small-dosage (0.1 mg/kg) triptolide treated group, moderate-dosage (0.2 mg/kg) triptolide treated group, large-dosage (0.4 mg/kg) triptolide treated group, propanediol (20%, 5 ml/kg) control group and dexamethasone (0.2 mg/kg) control group. The colitis rat model was induced by TNBS/ethanol. The rats were treated with the corresponding agent for 2 weeks, and white blood cell count was performed on the day before the rats were sacrificed. The colonic ulcerative surface area and thymus wet-dry ratio were calculated, superoxide dismutase (SOD) and myeloperoxidase (MPO) activity of the colonic mucosa were measured. The mucosal contents of Interleukin (IL)-1 and tumor necrisis factor (TNF)-α of colonic tissue in rats were detected by enzyme-linked immunosorbent assay (ELISA). Results: Remarkable efficacy of varying dosages of triptolide was observed in TNBS-induced colitis, with decrease of ulcerative surface area, thymus wet-dry ratio, MPO activity and increase of SOD activity. At the same time, the thymus was significantly regressed and white blood cell count diminished. Conclusions: The triptolide has noticeable therapeutic effects on TNBS/-induced experimental colitis in rats, but adverse effects have also occured when large dosage of triplide is used.

关 键 词：雷公藤内酯醇 大鼠 实验性结肠炎 IBD 细胞因子类 

分 类 号：R574.62[医药卫生—消化系统]