自体骨髓干细胞移植狗牙周缺损处引导组织再生的实验观察  

Effects of autogenous bone marrow stem cell transplantation on periodontal tissue regeneration in dogs

作　　者：欧龙[1] 马良[1] 王东胜[1] 罗芸[1] 袁志萍[1] 刘宏伟[1] 庞劲凡[1] 

机构地区：[1]解放军总医院口腔科,北京100853

出　　处：《现代口腔医学杂志》2005年第4期390-393,共4页Journal of Modern Stomatology

基　　金：国家自然科学基金(编号:39770798);国家教委留学回国人员科研启动基金(外教司留[1997]436号)资助课题

摘　　要：目的本文对应用自体骨髓干细胞移植引导组织再生的动物实验的观察进行评价。方法6只成年狗,实验组,对照组各18颗牙。分别在每条狗抽取骨髓1ml,在实验室内进行原代骨髓干细胞培养,培养液为内含15%小牛血清(FCS)和0.5%青-链霉素抗生素的a-MEM培养液。第1代细胞转移到18块大小为6×2mm2胶原膜上,约每张胶原膜上1×107个细胞,培养24小时后相差显微镜下观察细胞在膜上附着情况。在人工制造的牙周缺损中进行体外培养的自体骨髓干细胞移植结合GTR方法(实验组)和单纯GTR方法(对照组)。在6周后切片行牙周组织学观察。结果实验组新生牙槽骨新生牙周膜组织及新生牙骨质的修复再生的效果明显好于对照组(P<0.05),形成了的牙周结构,只是引导再生的牙周组织基本恢复到正常的牙周组织高度。实验组牙槽骨再生高度平均为4.50±0.13mm;对照组为3.09±0.28mm。结论应用自体骨髓干细胞移植结合e-pTFE膜引导牙周组织再生可促进牙周组织的再生、加快正常骨结构组织的建立并缩短修复再生时间。Objective To evaluate the effect of autogenous bone marrow stem cell transplantation on periodontal tissue regeneration.Methods Autogenous bone marrow stem cells from same dog were cultured with á-MEM. 1×107 cells of first passage were allowed to attach to the collagen membrane for 24 hours. The membrane-cells were transplanted into periodontal defect in the same dog. The defects were then covered with e-pTFE membranes. Defects covered with e-pTFE only, without membrane-cells, served as controls. Eighteen teeth from 6 dogs in each group were included in the study. The dogs were sacrificed at 6 weeks.Results New bone formation ( 4.50± 0.13mm) in the experimental group was significantly higher than that in the control group ( 3.09± 0.28mm,P< 0.05).Conclusion Autogenous bone marrow stem cell transplantation with guided tissue regeneration technique could enhance periodontal tissue regeneration in dogs.

关 键 词：引导组织再生 自体骨髓 牙周缺损 实验观察 引导牙周组织再生 狗 显微镜下观察 修复再生 组织学观察 牙周膜组织 牙槽骨再生 实验组 对照组 实验室内 人工制造 引导再生 培养液 抗生素 链霉素 小牛血 膜上 牙骨质 骨新生 

分 类 号：R781.4[医药卫生—口腔医学] R457.7[医药卫生—临床医学]